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MYL2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200494

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of… More details

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Product Overview

Online Datasheet in PDF
Product Name
MYL2, Monoclonal Antibody
Full Product Name
MYL2
Product Gene Name
anti-MYL2 antibody
Product Synonym Names
Slow cardiac myosin regulatory light chain 2; MLC2; CMH10; DKFZp779C0562; Slow cardiac myosin regulatory light chain 2; MYL2; Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2; MLC 2v; MYL 2; Cardiac ventricular myosin light chain 2; RLC of myosin; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2 ventricular cardiac muscle isoform; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT3B2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Purity/Purification
Protein-A Affinity Chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
160781
3D Structure
ModBase 3D Structure for P10916
Immunogen
Recombinant human MYL2 (1-166aa) purified from E Coli.
Conjugation
Unconjugated

Product Categories/Family for anti-MYL2 antibody

Cytoskeleton
Applications Tested/Suitable for anti-MYL2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-MYL2 antibody

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;
Lane 1.: Mouse Heart Tissue lysate
Mouse Anti-MYL2 Monoclonal Antibody – Cat# MBS200494

Flow Cytometry (FC/FACS) of anti-MYL2 antibody

Flow cytometry analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-MYL2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS200494

Product References and Citations for anti-MYL2 antibody

Macera MJ, et al., (1992) Genomics. 13(3):829-31. Poetter K, et al., (1996) Nat Genet. 13(1):63-9.

Other Notes

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL2 purchase

MBS200494 is an antibody targeting MYL2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL2. It may not necessarily be applicable to this product.

NCBI GI #
94981553
NCBI GeneID
4633
NCBI Accession #
NP_000423.2
UniProt Primary Accession #
P10916
UniProt Related Accession #
P10916
GenBank Nucleotide #
NP_000423.2
NCBI Official Full Name
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Official Synonym Full Names
myosin light chain 2
NCBI Official Symbol
MYL2
NCBI Official Synonym Symbols
MLC2; CMH10; MLC-2s/v
UniProt Protein Name
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family
Myosin regulatory
UniProt Gene Name
MYL2
UniProt Synonym Gene Names
MLC-2; MLC-2v
UniProt Entry Name
MLRV_HUMAN
NCBI Protein Information
myosin regulatory light chain 2, ventricular/cardiac muscle isoform

NCBI Summary for MYL2

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
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UniProt Comments for MYL2

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; post-embryonic development; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 10
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Research Articles on MYL2

1. R58Q of myosin regulatory light chain promotes an OFF state of the thick filaments that reduces the number of myosin head domains that are available for actin interaction and ATP utilization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYL2 antibody

 Reference Product  PubMed Publications
 MYH7 antibody  >28 publications with MYL2 and MYH7
 MYL3 antibody  >27 publications with MYL2 and MYL3
 TNNI3 antibody  >24 publications with MYL2 and TNNI3
 MYBPC3 antibody  >22 publications with MYL2 and MYBPC3
 TNNT2 antibody  >22 publications with MYL2 and TNNT2
 TPM1 antibody  >17 publications with MYL2 and TPM1
 TTN antibody  >4 publications with MYL2 and TTN

Pathways associated with anti-MYL2 antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-MYL2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >35 publications with MYL2 and Cardiovascular Diseases
 Heart Diseases Antibodies  >33 publications with MYL2 and Heart Diseases
 Cardiomyopathy, Hypertrophic Antibodies  >27 publications with MYL2 and Cardiomyopathy, Hypertrophic
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >11 publications with MYL2 and Cardiomyopathy, Hypertrophic, Familial
 Hypertrophy Antibodies  >10 publications with MYL2 and Hypertrophy
 Hypertension Antibodies  >5 publications with MYL2 and Hypertension
 Cardiomegaly Antibodies  >5 publications with MYL2 and Cardiomegaly
 Neoplasms Antibodies  >5 publications with MYL2 and Neoplasms
 Cardiomyopathy, Familial Hypertrophic, 10 Antibodies  >4 publications with MYL2 and Cardiomyopathy, Familial Hypertrophic, 10
 Inflammation Antibodies  >2 publications with MYL2 and Inflammation

Organs/Tissues associated with anti-MYL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >36 publications with MYL2 and Muscle
 Heart Antibodies  >29 publications with MYL2 and Heart
 Embryonic Tissue Antibodies  >4 publications with MYL2 and Embryonic Tissue
 Bone Antibodies  >3 publications with MYL2 and Bone
 Vascular Antibodies  >2 publications with MYL2 and Vascular
 Nerve Antibodies  >2 publications with MYL2 and Nerve
 Lymph Node Antibodies  >1 publications with MYL2 and Lymph Node
 Eye Antibodies  >1 publications with MYL2 and Eye
 Mouth Antibodies  >1 publications with MYL2 and Mouth
 Lung Antibodies  >1 publications with MYL2 and Lung
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