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MafK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200501

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locuscontrol regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by thetranscription factor NFE2 (nuclear factor… More details

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Product Overview

Online Datasheet in PDF
Product Name
MafK, Monoclonal Antibody
Full Product Name
MafK
Product Gene Name
anti-MafK antibody
Product Synonym Names
Transcription factor MafK; NFE2u; P18
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT2F7
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
OMIM
600197
3D Structure
ModBase 3D Structure for O60675
Immunogen
Recombinant human MAFK (1-156aa) purified from E. coli
Conjugation
Unconjugated
Product Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-MafK antibody

Epigenetics and Nuclear Signaling
Applications Tested/Suitable for anti-MafK antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MafK antibody
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-MafK antibody

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MafK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;
Mouse Anti-MafK Monoclonal Antibody – Cat# MBS200501

Product References and Citations for anti-MafK antibody

Oyake, T., et al. (1996) Mol Cell Biol 16(11): 6083-95.;;Toki, T., et al. (1997) Oncogene 14: 1901-1910.

Other Notes

Small volumes of anti-MafK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MafK purchase

MBS200501 is an antibody targeting MafK. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MafK. It may not necessarily be applicable to this product.

NCBI GI #
4505075
NCBI GeneID
7975
NCBI Accession #
NP_002351.1
UniProt Primary Accession #
O60675
UniProt Related Accession #
O60675
GenBank Nucleotide #
NP_002351.1
NCBI Official Full Name
transcription factor MafK
NCBI Official Synonym Full Names
MAF bZIP transcription factor K
NCBI Official Symbol
MAFK
NCBI Official Synonym Symbols
P18; NFE2U
UniProt Protein Name
Transcription factor MafK
UniProt Synonym Protein Names
Erythroid transcription factor NF-E2 p18 subunit
Protein Family
Transcription factor
UniProt Gene Name
MAFK
UniProt Entry Name
MAFK_HUMAN
NCBI Protein Information
transcription factor MafK

NCBI Summary for MafK

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]
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UniProt Comments for MafK

MAFK: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: nucleoplasm

Molecular Function: transcription factor activity

Biological Process: nervous system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; blood coagulation
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Research Articles on MafK

1. MAFK genetic polymorphisms are significantly associated with susceptibility to ulcerative colitis development in Japan.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MafK antibody

 Reference Product  PubMed Publications
 MAFG antibody  >45 publications with MafK and MAFG
 MAFF antibody  >30 publications with MafK and MAFF
 NFE2 antibody  >30 publications with MafK and NFE2
 NFE2L2 antibody  >28 publications with MafK and NFE2L2
 BACH1 antibody  >19 publications with MafK and BACH1
 BACH2 antibody  >8 publications with MafK and BACH2
 NFE2L3 antibody  >2 publications with MafK and NFE2L3
 NFE2L1 antibody  >1 publications with MafK and NFE2L1
 NGF antibody  >1 publications with MafK and NGF
 HBE1 antibody  >1 publications with MafK and HBE1

Pathways associated with anti-MafK antibody

 Products by Pathway  Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-MafK antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >12 publications with MafK and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with MafK and Liver Neoplasms
 Liver Neoplasms, Experimental Antibodies  >6 publications with MafK and Liver Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >4 publications with MafK and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >4 publications with MafK and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >3 publications with MafK and Kidney Diseases
 Inflammation Antibodies  >3 publications with MafK and Inflammation
 Nervous System Diseases Antibodies  >3 publications with MafK and Nervous System Diseases
 Precancerous Conditions Antibodies  >2 publications with MafK and Precancerous Conditions
 Necrosis Antibodies  >2 publications with MafK and Necrosis

Organs/Tissues associated with anti-MafK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >26 publications with MafK and Blood
 Embryonic Tissue Antibodies  >9 publications with MafK and Embryonic Tissue
 Bone Antibodies  >6 publications with MafK and Bone
 Eye Antibodies  >6 publications with MafK and Eye
 Brain Antibodies  >6 publications with MafK and Brain
 Placenta Antibodies  >6 publications with MafK and Placenta
 Kidney Antibodies  >5 publications with MafK and Kidney
 Pancreas Antibodies  >5 publications with MafK and Pancreas
 Lung Antibodies  >5 publications with MafK and Lung
 Heart Antibodies  >4 publications with MafK and Heart
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