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Alpha-2-Plasmin Inhibitor (a2PI) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2006582
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MBS2006582 is an antibody targeting a2PI. Also known as Polyclonal Antibody to Alpha-2-Plasmin Inhibitor (a2PI). Reactive in Mouse, Rat. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha-2-Plasmin Inhibitor (a2PI), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Alpha-2-Plasmin Inhibitor (a2PI)
Product Gene Name
anti-a2PI antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-VAT LSQLGLQELF QGPDLRGISE QNLVVSSVQH QSTMELSEAG VEAAAATSVA MNRMSLSSFT VNRPFLFFIM EDTIGVPLFV GSVRNPNPSA LPQLQEQRDS PDNRLIGQND KADFHGGKTF GPDLKLAPRM EEDYPQFSSP K
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against a2PI. It has been selected for its ability to recognize a2PI in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: a2PI (MBS2006582)
Biotin Conjugated Antibody: a2PI (MBS2001884)
Matching Pairs
Unconjugated Antibody: a2PI (MBS2006582)
Immunogen: a2PI (MBS2010885)
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Molecular Weight
54,972 Da
3D Structure
ModBase 3D Structure for Q61247
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant a2PI (Val348~Lys491) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038987
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-a2PI antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-a2PI antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-a2PI antibody

Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse a2PI Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
Rabbit Anti-a2PI Polyclonal Antibody – Cat# MBS2006582

Western Blot (WB) of anti-a2PI antibody

Western Blot: Sample: Mouse Serum; Primary Ab: 2ug/ml Rabbit Anti-Mouse a2PI Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
Rabbit Anti-a2PI Polyclonal Antibody, Western Blot (WB) – Cat# MBS2006582

Western Blot (WB) of anti-a2PI antibody

Western Blot: Sample: Mouse Liver lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse a2PI Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
Rabbit Anti-a2PI Polyclonal Antibody, Western Blot (WB) – Cat# MBS2006582

Western Blot (WB) of anti-a2PI antibody

Western Blot: Sample: Recombinant protein
Rabbit Anti-a2PI Polyclonal Antibody, Western Blot (WB) – Cat# MBS2006582

Immunohistochemistry (IHC) of anti-a2PI antibody

DAB staining on IHC-P. Samples: Mouse Tissue)
Rabbit Anti-a2PI Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006582

Other Notes

Small volumes of anti-a2PI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for a2PI. It may not necessarily be applicable to this product.

NCBI GI #
6679383
NCBI GeneID
18816
NCBI Accession #
NP_032904.1
UniProt Primary Accession #
Q61247
UniProt Related Accession #
Q61247
GenBank Nucleotide #
NM_008878.2
NCBI Official Full Name
alpha-2-antiplasmin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 2
NCBI Official Symbol
Serpinf2
NCBI Official Synonym Symbols
Pli; AI747498; Serpimf2
UniProt Protein Name
Alpha-2-antiplasmin
UniProt Synonym Protein Names
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
UniProt Gene Name
Serpinf2
UniProt Synonym Gene Names
Pli; Alpha-2-AP; Alpha-2-PI
NCBI Protein Information
alpha-2-antiplasmin

UniProt Comments for a2PI

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11|11 B5

Cellular Component: cell surface; extracellular region; extracellular space; fibrinogen complex

Molecular Function: peptidase inhibitor activity; protease binding; protein homodimerization activity; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; blood vessel morphogenesis; collagen fibril organization; negative regulation of fibrinolysis; negative regulation of peptidase activity; positive regulation of cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; regulation of blood vessel diameter by renin-angiotensin

Disease: Alpha-2-plasmin Inhibitor Deficiency
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Research Articles on a2PI

1. alpha2AP has a profibrotic effect probably not by the action as a plasmin inhibitor, and the blocking of alpha2AP exerts an antifibrotic effect in humans and mice with systemic sclerosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-a2PI antibody

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with anti-a2PI antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3 publications with a2PI and Liver Diseases
 Cardiovascular Diseases Antibodies  >2 publications with a2PI and Cardiovascular Diseases
 Kidney Diseases Antibodies  >1 publications with a2PI and Kidney Diseases
 Fibrosis Antibodies  >1 publications with a2PI and Fibrosis
 Neoplasms Antibodies  >1 publications with a2PI and Neoplasms
 Liver Neoplasms Antibodies  >1 publications with a2PI and Liver Neoplasms
 Inflammation Antibodies  >1 publications with a2PI and Inflammation
 Carcinoma, Hepatocellular Antibodies  >1 publications with a2PI and Carcinoma, Hepatocellular
 Blood Coagulation Disorders Antibodies  >1 publications with a2PI and Blood Coagulation Disorders

Organs/Tissues associated with anti-a2PI antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with a2PI and Liver
 Heart Antibodies  >1 publications with a2PI and Heart
 Kidney Antibodies  >1 publications with a2PI and Kidney
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