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BH3 Interacting Domain Death Agonist (BID) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2006815
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MBS2006815 is an antibody targeting Bid. Also known as Polyclonal Antibody to BH3 Interacting Domain Death Agonist (Bid). Reactive in Mouse, Rat. Host: Rabbit. More details

$200 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BH3 Interacting Domain Death Agonist (Bid), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to BH3 Interacting Domain Death Agonist (Bid)
Product Gene Name
anti-Bid antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDSEVSNGSG LGAEHITDLL VFGFLQSSGC TRQELEVLGR ELPVQAYWEA DLEDELQTDG SQASRSFNQG RIEPDSESQE EIIHNIARHL AQIGDEMDHN IQPTLVRQLA AQFMNGSLSE EDKRNCLAKA LDEVKTAFPR DMENDKAMLI MTMLLAKKVA SHAPSLLRDV FHTTVNFINQ NLFSYVRNLV RNEMD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
0.2mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against Bid. It has been selected for its ability to recognize Bid in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: Bid (MBS2006815)
Biotin Conjugated Antibody: Bid (MBS2001531)
Matching Pairs
Unconjugated Antibody: Bid (MBS2006815)
Immunogen: Bid (MBS2010036)
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Molecular Weight
21,952 Da
3D Structure
ModBase 3D Structure for P70444
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant Bid (Met1~Asp195) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045234
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-Bid antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-Bid antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-Bid antibody

Western Blot: Sample: Mouse Blood Cells lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse Bid Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-BID Polyclonal Antibody – Cat# MBS2006815

Western Blot (WB) of anti-Bid antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-BID Polyclonal Antibody, Western Blot (WB) – Cat# MBS2006815

Immunohistochemistry (IHC) of anti-Bid antibody

DAB staining on fromalin fixed paraffin- embedded spleen tissue)
Rabbit Anti-BID Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006815

Immunohistochemistry (IHC) of anti-Bid antibody

DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
Rabbit Anti-BID Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006815

Other Notes

Small volumes of anti-Bid antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Bid. It may not necessarily be applicable to this product.

NCBI GI #
31542228
NCBI GeneID
12122
NCBI Accession #
NP_031570.2
UniProt Primary Accession #
P70444
UniProt Secondary Accession #
Q99M39
UniProt Related Accession #
P70444
GenBank Nucleotide #
NM_007544.3
NCBI Official Full Name
BH3-interacting domain death agonist
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
Bid
NCBI Official Synonym Symbols
AI875481; AU022477; 2700049M22Rik
UniProt Protein Name
BH3-interacting domain death agonist
UniProt Synonym Protein Names
p22 BID; BID
Protein Family
BH3-interacting domain death agonist
UniProt Gene Name
Bid
UniProt Synonym Gene Names
BID
NCBI Protein Information
BH3-interacting domain death agonist

UniProt Comments for Bid

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 6 F1|6 57.02 cM

Cellular Component: cytoplasm; cytosol; extracellular exosome; membrane; mitochondrion

Molecular Function: protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; apoptosis; apoptotic mitochondrial changes; establishment of protein localization to membrane; negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage; organelle ATP synthesis coupled electron transport; positive regulation of apoptosis; positive regulation of apoptotic signaling pathway; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of fibroblast apoptotic process; positive regulation of mitochondrial membrane potential; positive regulation of protein homooligomerization; positive regulation of protein oligomerization; positive regulation of release of cytochrome c from mitochondria; protein homooligomerization; protein targeting to mitochondrion; regulation of apoptosis; regulation of cell proliferation; regulation of G1/S transition of mitotic cell cycle; regulation of mitochondrial membrane permeability involved in apoptotic process; regulation of protein oligomerization; release of cytochrome c from mitochondria; signal transduction in response to DNA damage
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Research Articles on Bid

1. Cleaved Bid did not permeabilize the membrane, but lowered the membrane breakthrough force.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Bid antibody

 Reference Product  PubMed Publications
 Casp8 antibody  >1204 publications with Bid and Casp8
 Bax antibody  >1150 publications with Bid and Bax
 Bcl2l1 antibody  >344 publications with Bid and Bcl2l1
 Bak1 antibody  >117 publications with Bid and Bak1
 Bcl2 antibody  >109 publications with Bid and Bcl2
 Gzmb antibody  >62 publications with Bid and Gzmb
 Gzmf antibody  >1 publications with Bid and Gzmf
 Gzmc antibody  >1 publications with Bid and Gzmc

Pathways associated with anti-Bid antibody

 Products by Pathway  Pathway Diagram
 Activation And Oligomerization Of BAK Protein Pathway antibodies  Activation And Oligomerization Of BAK Protein Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Activation, Translocation And Oligomerization Of BAX Pathway antibodies  Activation, Translocation And Oligomerization Of BAX Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram

Diseases associated with anti-Bid antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >893 publications with Bid and Cardiovascular Diseases
 Necrosis Antibodies  >694 publications with Bid and Necrosis
 Lung Diseases Antibodies  >693 publications with Bid and Lung Diseases
 Gastrointestinal Diseases Antibodies  >654 publications with Bid and Gastrointestinal Diseases
 Nervous System Diseases Antibodies  >630 publications with Bid and Nervous System Diseases
 Pain Antibodies  >469 publications with Bid and Pain
 Heart Diseases Antibodies  >441 publications with Bid and Heart Diseases
 Adenocarcinoma Antibodies  >367 publications with Bid and Adenocarcinoma
 Diarrhea Antibodies  >354 publications with Bid and Diarrhea
 Hypertension Antibodies  >322 publications with Bid and Hypertension

Organs/Tissues associated with anti-Bid antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1910 publications with Bid and Blood
 Liver Antibodies  >591 publications with Bid and Liver
 Heart Antibodies  >543 publications with Bid and Heart
 Lung Antibodies  >537 publications with Bid and Lung
 Brain Antibodies  >393 publications with Bid and Brain
 Kidney Antibodies  >287 publications with Bid and Kidney
 Skin Antibodies  >253 publications with Bid and Skin
 Bone Antibodies  >213 publications with Bid and Bone
 Muscle Antibodies  >195 publications with Bid and Muscle
 Stomach Antibodies  >190 publications with Bid and Stomach
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