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Alpha-2-Macroglobulin (a2M) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2006946
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MBS2006946 is an antibody targeting a2M. Also known as Polyclonal Antibody to Alpha-2-Macroglobulin (a2M). Reactive in Mouse, Rat. Host: Rabbit. More details

$200 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha-2-Macroglobulin (a2M), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Alpha-2-Macroglobulin (a2M)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- VFRPNSG LPATRALLSV MASPQSLCGL RAVDQSVLLM KPETELSASL IYDLLPVKDL TGFPQGADQR EEDTNGCVKQ NDTYINGILY SPVQNTNEED MYGFLKDMGL KVFTNSNIRK PKVCERLRDN KGIPAAYHLV SQSHMDAFLE SSESPTETRR SYFP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against a2M. It has been selected for its ability to recognize a2M in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: a2M (MBS2006946)
Immunogen: a2M (MBS2010061)
Matching Pairs
Unconjugated Antibody: a2M (MBS2006946)
APC-CY7 Conjugated Antibody: a2M (MBS2050537)
Molecular Weight
163,785 Da
3D Structure
ModBase 3D Structure for P06238
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant a2M (Val574~Pro734) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050545
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-a2M antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-a2M antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-a2M antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-a2M Polyclonal Antibody – Cat# MBS2006946

Immunohistochemistry (IHC) of anti-a2M antibody

DABstainingonIHC-P.Samples:RatTissue)
Rabbit Anti-a2M Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006946

Other Notes

Small volumes of anti-a2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for a2M. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
A2m
NCBI Official Synonym Symbols
Mam; A2m1; A2mb; A2maa; A2MAC1
UniProt Protein Name
Alpha-2-macroglobulin
Protein Family
UniProt Gene Name
A2m
UniProt Synonym Gene Names
A2m1; Alpha-2-M
NCBI Protein Information
alpha-2-macroglobulin

NCBI Summary for a2M

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

UniProt Comments for a2M

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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