3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Hexokinase 2 (HXK2) Active Protein

Name: HXK2 Active Protein; Hexokinase 2; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS201056
Price: 285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201056

Hexokinase2 is one of four highly homologous hexokinase isoforms in mammalian cells. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Hexokinase 2 (HXK2), Active Protein

Full Product Name

Recombinant Hexokinase 2

Product Gene Name

HXK2 active protein

Product Synonym Names

HKII

Sequence

MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR

Host

E Coli

Form/Format

Liquid; 20mM Tris-HCl Buffer (pH8.0) Containing 10% Glycerol, 0.1M NaCl, 0.1mM PMSF.

Purity/Purification

>90% by SDS-PAGE

Concentration

1mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Molecular Weight

104.5 kDa (937aa)

Sequence Positions

1-917aa

OMIM

601125

3D Structure

ModBase 3D Structure for P52789

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Endotoxin

<1.0 EU per 1ug of protein (determined by LAL method)

Tag

His-Tag

Biological Activity

Specific activity is > 40,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.

Product Categories/Family for HXK2 active protein

Signal Transduction; Metabolism; Cancer

Applications Tested/Suitable for HXK2 active protein

Enzyme Activity, SDS-PAGE

Product References and Citations for HXK2 active protein

Travis SM. et al. (1997) FEBS Lett. 412(3):415-9. Brautigan D.L. et al. (2005) Biochemistry 44(33):11067-73.

Other Notes

Small volumes of HXK2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HXK2 purchase

MBS201056 is an active protein targeting HXK2. Also known as Recombinant Hexokinase 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for HXK2. It may not necessarily be applicable to this product.

NCBI GI #

39963174

NCBI GeneID

3099

NCBI Accession #

AAH64369.1

UniProt Primary Accession #

P52789

UniProt Related Accession #

P52789

NCBI Official Full Name

Hexokinase 2

NCBI Official Synonym Full Names

hexokinase 2

NCBI Official Symbol

HK2

NCBI Official Synonym Symbols

HKII; HXK2

UniProt Protein Name

Hexokinase-2

UniProt Synonym Protein Names

Hexokinase type II; HK II; Muscle form hexokinase

Protein Family

Hexokinase

UniProt Gene Name

HK2

UniProt Synonym Gene Names

HK II

UniProt Entry Name

HXK2_HUMAN

NCBI Protein Information

hexokinase-2

NCBI Summary for HXK2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

UniProt Comments for HXK2

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Kinase, other; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; cell glucose homeostasis; regulation of glucose import; pathogenesis; glucose transport; transmembrane transport

Research Articles on HXK2

1. Results found that hepatocellular carcinoma (HCC) patients with increased HK-II expression in the TCGA database showed poor overall survival. These results suggest that HK-II expression might be a prognostic factor in HCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HXK2 active protein

Reference Product	PubMed Publications
GPI active protein	>4 publications with HXK2 and GPI
PFKM active protein	>2 publications with HXK2 and PFKM
PFKL active protein	>1 publications with HXK2 and PFKL
C12orf5 active protein	>1 publications with HXK2 and C12orf5
PFKP active protein	>1 publications with HXK2 and PFKP

Pathways associated with HXK2 active protein

Products by Pathway	Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Butirosin And Neomycin Biosynthesis Pathway antibodies	Butirosin And Neomycin Biosynthesis Pathway Diagram
Butirosin And Neomycin Biosynthesis Pathway antibodies	Butirosin And Neomycin Biosynthesis Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram

Diseases associated with HXK2 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>208 publications with HXK2 and Neoplasms
Kidney Diseases Antibodies	>41 publications with HXK2 and Kidney Diseases
Hyperplasia Antibodies	>39 publications with HXK2 and Hyperplasia
Fibrosis Antibodies	>27 publications with HXK2 and Fibrosis
Breast Neoplasms Antibodies	>21 publications with HXK2 and Breast Neoplasms
Death Antibodies	>20 publications with HXK2 and Death
Cardiovascular Diseases Antibodies	>14 publications with HXK2 and Cardiovascular Diseases
Drug Toxicity Antibodies	>12 publications with HXK2 and Drug Toxicity
Neoplasms, Experimental Antibodies	>10 publications with HXK2 and Neoplasms, Experimental
Disease Models, Animal Antibodies	>9 publications with HXK2 and Disease Models, Animal

Organs/Tissues associated with HXK2 active protein

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>237 publications with HXK2 and Prostate
Blood Antibodies	>125 publications with HXK2 and Blood
Kidney Antibodies	>97 publications with HXK2 and Kidney
Muscle Antibodies	>26 publications with HXK2 and Muscle
Liver Antibodies	>20 publications with HXK2 and Liver
Brain Antibodies	>19 publications with HXK2 and Brain
Heart Antibodies	>15 publications with HXK2 and Heart
Embryonic Tissue Antibodies	>14 publications with HXK2 and Embryonic Tissue
Bone Antibodies	>12 publications with HXK2 and Bone
Lung Antibodies	>10 publications with HXK2 and Lung