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Hydroxyacid Oxidase-1/HAO-1 (HAO-1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201135

Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Hydroxyacid Oxidase-1/HAO-1, Active Protein
Full Product Name
Recombinant Hydroxyacid Oxidase-1/HAO-1
Product Gene Name
Product Synonym Names
(S)-2-hydroxy-acid oxidase; EC 1.1.3.15; Glycolate oxidase; GOX; GOX1MGC142227; GOXMGC142225; HAO1; HAO-1; HAOX1; hydroxyacid oxidase (glycolate oxidase) 1; hydroxyacid oxidase 1; Hydroxyacid Oxidase1; Hydroxyacid Oxidase-1
Sequence
MGSSHHHHHH SSGLVPRGSH MGS MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI
Host
E Coli
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 10% Glycerol.
Purity/Purification
>90% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles
Molecular Weight
43.4 kDa (393aa), confirmed by MALDI-TOF
Sequence Positions
1-370aa
3D Structure
ModBase 3D Structure for Q9WU19
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Biological Activity
Specific activity is > 1,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.

Product Categories/Family for HAO-1 active protein

Applications Tested/Suitable for HAO-1 active protein
SDS-PAGE, Enzyme Activity

SDS-Page of HAO-1 active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
HAO-1 Active Protein – Cat# MBS201135

Product References and Citations for HAO-1 active protein

Pennati A., et al. (2009) J. Biol. Chem. 284(45):31214-22. Murray MS., et al. (2008) Biochemistry. 47(8):2439-49.

Other Notes

Small volumes of HAO-1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAO-1 purchase

MBS201135 is an active protein targeting HAO-1. Also known as Recombinant Hydroxyacid Oxidase-1/HAO-1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for HAO-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hydroxyacid oxidase 1
NCBI Official Synonym Full Names
hydroxyacid oxidase 1, liver
NCBI Official Symbol
Hao1
NCBI Official Synonym Symbols
GOX; Gox1; Hao-1
UniProt Protein Name
Hydroxyacid oxidase 1
UniProt Synonym Protein Names
Glycolate oxidase; GOX
UniProt Gene Name
Hao1
UniProt Synonym Gene Names
Gox1; Hao-1; HAOX1; GOX
UniProt Entry Name
HAOX1_MOUSE
NCBI Protein Information
hydroxyacid oxidase 1

UniProt Comments for HAO-1

HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15

Cellular Component: peroxisome

Molecular Function: glycolate oxidase activity; FMN binding; oxidoreductase activity; catalytic activity; receptor binding; (S)-2-hydroxy-acid oxidase activity

Biological Process: glycolate catabolic process; fatty acid alpha-oxidation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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