3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

IL-1ra/IL-1F3 Active Protein

Name: IL-1ra/IL-1F3 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS201149
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201149

Interleukin 1 receptor antagonist (IL1RN), also known as IL1-RA, is a member of the interleukin 1 cytokine family. IL1RN may represent a reservoir of IL-1 inhibitors, released upon cell death, whose function is no limit the pro-inflammatory signals but most… More details

$220 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL-1ra/IL-1F3, Active Protein

Full Product Name

Recombinant IL-1ra/IL-1F3

Product Gene Name

IL-1ra/IL-1F3 active protein

Product Synonym Names

Interleukin 1 receptor antagonist; F630041P17Rik; IL-1ra

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHM RPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL QGPNIKLEEK IDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEED KRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ

Host

E Coli

Form/Format

Liquid; In 20mM Tris-HCl Buffer (pH 8.0) Containing 0.1M NaCl, 10% Glycerol.

Purity/Purification

>95% by SDS-PAGE

Concentration

1mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Molecular Weight

20 kDa (177aa), confirmed by MALDI-TOF

Sequence Positions

27-178aa

3D Structure

ModBase 3D Structure for P25085

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Mouse

Endotoxin

<1EU per 1ug of protein (determined by LAL method)

Tag

His-Tag

Biological Activity

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/ml with Mouse IL-1 alpha.

Product Categories/Family for IL-1ra/IL-1F3 active protein

Hormones & Cytokines

Applications Tested/Suitable for IL-1ra/IL-1F3 active protein

SDS-PAGE, Bioactivity

Product References and Citations for IL-1ra/IL-1F3 active protein

Steinkasserer A., et al. (1992) Genomics. 13(3):654-7. Langdahl BL., et al. (2000) J Bone Miner Res. 15(3): 403-14.

Other Notes

Small volumes of IL-1ra/IL-1F3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-1ra/IL-1F3 purchase

MBS201149 is an active protein targeting IL-1ra/IL-1F3. Also known as Recombinant IL-1ra/IL-1F3. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-1ra/IL-1F3. It may not necessarily be applicable to this product.

NCBI GI #

89257344

NCBI GeneID

16181

NCBI Accession #

NP_001034790.1

UniProt Primary Accession #

P25085

UniProt Related Accession #

P25085

GenBank Nucleotide #

NP_001034790.1

NCBI Official Full Name

interleukin-1 receptor antagonist protein isoform 2

NCBI Official Synonym Full Names

interleukin 1 receptor antagonist

NCBI Official Symbol

Il1rn

NCBI Official Synonym Symbols

IL-1ra; F630041P17Rik

UniProt Protein Name

Interleukin-1 receptor antagonist protein

UniProt Synonym Protein Names

IL1 inhibitor

UniProt Gene Name

Il1rn

UniProt Synonym Gene Names

Il-1ra; IL-1RN; IL-1ra; IRAP

UniProt Entry Name

IL1RA_MOUSE

NCBI Protein Information

interleukin-1 receptor antagonist protein

UniProt Comments for IL-1ra/IL-1F3

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; vesicle

Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity

Biological Process: positive regulation of JNK activity; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; cytokine and chemokine mediated signaling pathway; response to glucocorticoid stimulus; fever; sensory perception of pain; memory; insulin secretion; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis

Research Articles on IL-1ra/IL-1F3

1. these data reveal that IL-1Ra and IL-36Ra have opposing effects on the outcome of Pseudomonas aeruginosa keratitis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IL-1ra/IL-1F3 active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
IL-1 Signaling Pathway antibodies	IL-1 Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-1 Signaling Pathway antibodies	Interleukin-1 Signaling Pathway Diagram
Signaling By Interleukins Pathway antibodies	Signaling By Interleukins Pathway Diagram

Diseases associated with IL-1ra/IL-1F3 active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>778 publications with IL-1ra/IL-1F3 and Necrosis
Inflammation Antibodies	>697 publications with IL-1ra/IL-1F3 and Inflammation
Arthritis, Rheumatoid Antibodies	>302 publications with IL-1ra/IL-1F3 and Arthritis, Rheumatoid
Cardiovascular Diseases Antibodies	>190 publications with IL-1ra/IL-1F3 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>165 publications with IL-1ra/IL-1F3 and Disease Models, Animal
Skin Diseases Antibodies	>126 publications with IL-1ra/IL-1F3 and Skin Diseases
Lung Diseases Antibodies	>111 publications with IL-1ra/IL-1F3 and Lung Diseases
Pain Antibodies	>82 publications with IL-1ra/IL-1F3 and Pain
Kidney Diseases Antibodies	>80 publications with IL-1ra/IL-1F3 and Kidney Diseases
Heart Diseases Antibodies	>76 publications with IL-1ra/IL-1F3 and Heart Diseases

Organs/Tissues associated with IL-1ra/IL-1F3 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1072 publications with IL-1ra/IL-1F3 and Blood
Skin Antibodies	>127 publications with IL-1ra/IL-1F3 and Skin
Bone Marrow Antibodies	>67 publications with IL-1ra/IL-1F3 and Bone Marrow
Stomach Antibodies	>51 publications with IL-1ra/IL-1F3 and Stomach
Connective Tissue Antibodies	>50 publications with IL-1ra/IL-1F3 and Connective Tissue
Spleen Antibodies	>43 publications with IL-1ra/IL-1F3 and Spleen
Intestine Antibodies	>40 publications with IL-1ra/IL-1F3 and Intestine
Eye Antibodies	>38 publications with IL-1ra/IL-1F3 and Eye
Vagina Antibodies	>11 publications with IL-1ra/IL-1F3 and Vagina
Thymus Antibodies	>7 publications with IL-1ra/IL-1F3 and Thymus