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SPARC Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201167

SPARC, also known as osteonectin, is a member of secreted matricellular proteins. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. This protein regulates cell… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SPARC, Recombinant Protein
Full Product Name
Recombinant SPARC
Product Gene Name
SPARC recombinant protein
Product Synonym Names
SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; OI17
Sequence
APQQE AL PDETEVVEET VAEVTEVSVG ANPVQVEVGE FDDGAEETEE EVVAENPCQN HHCKHGKVCE LDENNTPMCV CQDPTSCPAP IGEFEKVCSN DNKTFDSSCH FFATKCTLEG TKKGHKLHLD YIGPCKYIPP CLDSELTEFP LRMRDWLKNV LVTLYERDED NNLLTEKQKL RVKKIHENEK RLEAGDHPVE LLARDFEKNY NMYIFPVHWQ FGQLDQHPID GYLSHTELAP LRAPLIPMEH CTTRFFETCD LDNDKYIALD EWAGCFGIKQ KDIDKDLVI< H HHHHH>
Host
Baculovirus
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Purity/Purification
> 90% by SDS - PAGE
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoidrepeated freezing and thawing cycles.
Molecular Weight
33.8kDa (295aa)
Sequence Positions
18-303aa
OMIM
182120
3D Structure
ModBase 3D Structure for P09486
Tag
His-Tag
Predicted nterm
Ala
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)

Product Categories/Family for SPARC recombinant protein

Cell Cycle
Applications Tested/Suitable for SPARC recombinant protein
SDS-PAGE

SDS-Page of SPARC recombinant protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SPARC Recombinant Protein – Cat# MBS201167

Product References and Citations for SPARC recombinant protein

McDonald LT., et al. (2018) Am J Physiol Heart Circ Physiol. 315(1):H92-H100.;; Wang B., et al. (2018) J Biol Regul Homeost Agents. 32:1525-1531.

Other Notes

Small volumes of SPARC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPARC purchase

MBS201167 is a recombinant protein targeting SPARC. Also known as Recombinant SPARC. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for SPARC. It may not necessarily be applicable to this product.

NCBI GI #
338325
NCBI GeneID
6678
NCBI Accession #
AAA60993.1
UniProt Primary Accession #
P09486
UniProt Related Accession #
P09486
NCBI Official Full Name
osteonectin
NCBI Official Synonym Full Names
secreted protein acidic and cysteine rich
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON; OI17; BM-40
UniProt Protein Name
SPARC
UniProt Synonym Protein Names
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Protein Family
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
NCBI Protein Information
SPARC

NCBI Summary for SPARC

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
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UniProt Comments for SPARC

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to peptide hormone stimulus; receptor-mediated endocytosis; response to gravity; platelet activation; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii
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Research Articles on SPARC

1. angiogenesis of lung cancer may be related to the interaction of SPARC, TGFbeta1 and Endoglin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPARC recombinant protein

 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >61 publications with SPARC and TGFB1
 COL1A1 recombinant protein  >24 publications with SPARC and COL1A1
 EGF recombinant protein  >18 publications with SPARC and EGF
 SERPINE1 recombinant protein  >15 publications with SPARC and SERPINE1
 FN1 recombinant protein  >11 publications with SPARC and FN1
 TIMP1 recombinant protein  >4 publications with SPARC and TIMP1
 PLG recombinant protein  >1 publications with SPARC and PLG

Pathways associated with SPARC recombinant protein

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with SPARC recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >546 publications with SPARC and Neoplasms
 Cardiovascular Diseases Antibodies  >97 publications with SPARC and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >95 publications with SPARC and Adenocarcinoma
 Inflammation Antibodies  >92 publications with SPARC and Inflammation
 Fibrosis Antibodies  >86 publications with SPARC and Fibrosis
 Brain Diseases Antibodies  >68 publications with SPARC and Brain Diseases
 Breast Neoplasms Antibodies  >59 publications with SPARC and Breast Neoplasms
 Necrosis Antibodies  >57 publications with SPARC and Necrosis
 Heart Diseases Antibodies  >54 publications with SPARC and Heart Diseases
 Neoplasms, Experimental Antibodies  >43 publications with SPARC and Neoplasms, Experimental

Organs/Tissues associated with SPARC recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1004 publications with SPARC and Bone
 Connective Tissue Antibodies  >423 publications with SPARC and Connective Tissue
 Blood Antibodies  >305 publications with SPARC and Blood
 Vascular Antibodies  >274 publications with SPARC and Vascular
 Bone Marrow Antibodies  >181 publications with SPARC and Bone Marrow
 Embryonic Tissue Antibodies  >166 publications with SPARC and Embryonic Tissue
 Muscle Antibodies  >165 publications with SPARC and Muscle
 Mouth Antibodies  >147 publications with SPARC and Mouth
 Brain Antibodies  >137 publications with SPARC and Brain
 Skin Antibodies  >112 publications with SPARC and Skin
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