3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Carboxyl Ester Lipase/CEL (CEL) Active Protein

Name: CEL Active Protein; Carboxyl Ester Lipase/CEL; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS201170
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201170

CEL, also known as bile salt-activated lipase, is one of the Type-B carboxyl esterase/lipase family of enzymes. It is an enzyme produced by the adult pancreas and aids in the digestion of fats. Also, it catalyzes fat and vitamin absorption and acts in concert… More details

$290 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Carboxyl Ester Lipase/CEL, Active Protein

Full Product Name

Recombinant Carboxyl Ester Lipase/CEL

Product Gene Name

CEL active protein

Product Synonym Names

Cel; 1810036E18Rik; BAL; BSSL; Carboxyl ester lipase; Cholesterol esterase; Pancreaticlysophospholipase; Sterol esterase; Lip1; Bile salt-activated lipase; Bile salt-stimulated lipase

Sequence

AKLGAVYTEG GFVEGVNKKL SLLGGDSVDI FKGIPFATAK TLENPQRHPG WQGTLKATNF KKRCLQATIT QDNTYGQEDC LYLNIWVPQG RKQVSHNLPV MVWIYGGAFL MGSGQGANFL KNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG NFGLRDQHMA IAWVKRNIAA FGGDPDNITI FGESAGAASV SLQTLSPYNK GLIRRAISQS GMALSPWAIQ KNPLFWAKTI AKKVGCPTED TGKMAACLKI TDPRALTLAY KLPVKKQEYP VVHYLAFIPV IDGDFIPDDP INLYNNTADI DYIAGINNMD GHLFATIDVP AVDKTKQTVT EEDFYRLVSG HTVAKGLKGA QATFDIYTES WAQDPSQENM KKTVVAFETD VLFLIPTEIA LAQHKAHAKS AKTYSYLFSH PSRMPIYPKW MGADHADDLQ YVFGKPFATP LGYRPQDRAV SKAMIAYWTN FARSGDPNMG NSPVPTHWYP YTLENGNYLD ITKTITSASM KEHLREKFLK FWAVTFEVLP TVTGDQDTLT PPEDDSEVAP DPPSDDSQVV PVPPTDDSVE AQMPATIGF< H HHHHH>

Host

Baculovirus

Form/Format

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Purity/Purification

> 90% by SDS - PAGE

Concentration

0.5mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles

Molecular Weight

64.5kDa (585aa)

Sequence Positions

21-599aa

3D Structure

ModBase 3D Structure for Q64285

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Tag

His-Tag

Biological Activity

Specific activity is > 100,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Product Categories/Family for CEL active protein

Cell Cycle

Applications Tested/Suitable for CEL active protein

SDS-PAGE; Enzyme Activity

Product References and Citations for CEL active protein

Xiao X., et al. (2016) J Biol Chem. 291:23224-23236.;; Shindo K., et al. (2017) Oncotarget. 8:50824-50831.

Other Notes

Small volumes of CEL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CEL purchase

MBS201170 is an active protein targeting CEL. Also known as Recombinant Carboxyl Ester Lipase/CEL. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for CEL. It may not necessarily be applicable to this product.

NCBI GI #

1046363

NCBI GeneID

12613

NCBI Accession #

NP_034015

UniProt Primary Accession #

Q64285

UniProt Related Accession #

Q64285

NCBI Official Full Name

cholesterol esterase

NCBI Official Synonym Full Names

carboxyl ester lipase

NCBI Official Symbol

Cel

NCBI Official Synonym Symbols

BAL; 1810036E18Rik

UniProt Protein Name

Bile salt-activated lipase

UniProt Synonym Protein Names

Bile salt-stimulated lipase; BSSL; Carboxyl ester lipase; Cholesterol esterase; Pancreatic lysophospholipase; Sterol esterase

Protein Family

Bile salt-activated lipase

UniProt Gene Name

Cel

UniProt Synonym Gene Names

Lip1; BAL; BSSL

UniProt Entry Name

CEL_MOUSE

NCBI Protein Information

bile salt-activated lipase

UniProt Comments for CEL

CEL: Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8); also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid Metabolism - glycerolipid; Lipase; Lipid Metabolism - steroid biosynthesis; Secreted; EC 3.1.1.3; EC 3.1.1.13

Cellular Component: Golgi apparatus; extracellular space; protein complex; rough endoplasmic reticulum; intracellular membrane-bound organelle; cytoplasm; extracellular region; cytosol; lipid raft

Molecular Function: sterol esterase activity; retinyl-palmitate esterase activity; triacylglycerol lipase activity; hydrolase activity; protein complex binding; glycosphingolipid binding; lysophospholipase activity

Biological Process: retinol metabolic process; ceramide catabolic process; lipid metabolic process; pancreatic juice secretion; cholesterol catabolic process; lipid catabolic process

Research Articles on CEL

1. BSSL is a key player in the inflammatory process of arthritis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CEL active protein

Products by Pathway	Pathway Diagram
Acylglycerol Degradation Pathway antibodies	Acylglycerol Degradation Pathway Diagram
Acylglycerol Degradation Pathway antibodies	Acylglycerol Degradation Pathway Diagram
Digestion Of Dietary Lipid Pathway antibodies	Digestion Of Dietary Lipid Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with CEL active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>210 publications with CEL and Neoplasms
Nervous System Diseases Antibodies	>148 publications with CEL and Nervous System Diseases
Brain Diseases Antibodies	>88 publications with CEL and Brain Diseases
Death Antibodies	>75 publications with CEL and Death
Inflammation Antibodies	>54 publications with CEL and Inflammation
Liver Diseases Antibodies	>40 publications with CEL and Liver Diseases
Necrosis Antibodies	>40 publications with CEL and Necrosis
Kidney Diseases Antibodies	>40 publications with CEL and Kidney Diseases
Neoplasms, Experimental Antibodies	>29 publications with CEL and Neoplasms, Experimental
Anemia Antibodies	>23 publications with CEL and Anemia

Organs/Tissues associated with CEL active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>93 publications with CEL and Liver
Pancreas Antibodies	>76 publications with CEL and Pancreas
Lung Antibodies	>53 publications with CEL and Lung
Muscle Antibodies	>46 publications with CEL and Muscle
Stomach Antibodies	>26 publications with CEL and Stomach
Embryonic Tissue Antibodies	>19 publications with CEL and Embryonic Tissue
Mammary Gland Antibodies	>11 publications with CEL and Mammary Gland