3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Arginase 1/ARG1 (ARG1) Active Protein

Name: ARG1 Active Protein; Arginase 1/ARG1; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS201187
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201187

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution,… More details

$280 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Arginase 1/ARG1, Active Protein

Full Product Name

Arginase 1/ARG1

Product Gene Name

ARG1 active protein

Product Synonym Names

Arginase-1 liver; Arginase-1; liver; Arginase-1; liver A I; Al; ARG 1; ARG1; Arginase 1; Arginase liver; Arginase type I; Arginase1; Liver type arginase; Type I arginase.

Sequence

MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PK

Host

E coli

Form/Format

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

Purity/Purification

>85% by SDS - PAGE

Concentration

0.5mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Molecular Weight

35.8kDa (330aa), confirmed by MALDI-TOF

Sequence Positions

1-322aa

OMIM

207800

3D Structure

ModBase 3D Structure for P05089

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag

His-Tag

Biological activity

Specific activity is > 150,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C.

Product Categories/Family for ARG1 active protein

Metabolism

Applications Tested/Suitable for ARG1 active protein

SDS-PAGE, Enzyme Activity

Product References and Citations for ARG1 active protein

Wu G., et al (1998) The Biochemical journal 336 (Pt 1): 1-17.; Iyer RK., et al (2002) Molecular and cellular biology 22 (13): 4491-8.

Other Notes

Small volumes of ARG1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARG1 purchase

MBS201187 is an active protein targeting ARG1. Also known as Arginase 1/ARG1. Host: E coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ARG1. It may not necessarily be applicable to this product.

NCBI GI #

10947139

NCBI GeneID

383

NCBI Accession #

NP_000036

UniProt Primary Accession #

P05089

UniProt Related Accession #

P05089

GenBank Nucleotide #

NP_000036.2

NCBI Official Full Name

arginase-1 isoform 2

NCBI Official Synonym Full Names

arginase 1

NCBI Official Symbol

ARG1

UniProt Protein Name

Arginase-1

UniProt Synonym Protein Names

Liver-type arginase; Type I arginase

Protein Family

Arginase

UniProt Gene Name

ARG1

UniProt Entry Name

ARGI1_HUMAN

NCBI Protein Information

arginase-1

NCBI Summary for ARG1

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for ARG1

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; EC 3.5.3.1

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; liver development; response to amino acid stimulus; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; response to amine stimulus; urea cycle; lung development

Disease: Argininemia

Research Articles on ARG1

1. ARG1 was significantly up-regulated in acute myocardial infarction patients compared to controls.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ARG1 active protein

Reference Product	PubMed Publications
ARG2 active protein	>28 publications with ARG1 and ARG2
NOS1 active protein	>5 publications with ARG1 and NOS1

Pathways associated with ARG1 active protein

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
IL4-mediated Signaling Events Pathway antibodies	IL4-mediated Signaling Events Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with ARG1 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>62 publications with ARG1 and Inflammation
Neoplasms Antibodies	>59 publications with ARG1 and Neoplasms
Disease Models, Animal Antibodies	>36 publications with ARG1 and Disease Models, Animal
Nervous System Diseases Antibodies	>35 publications with ARG1 and Nervous System Diseases
Immune System Diseases Antibodies	>31 publications with ARG1 and Immune System Diseases
Necrosis Antibodies	>22 publications with ARG1 and Necrosis
Pain Antibodies	>20 publications with ARG1 and Pain
Cardiovascular Diseases Antibodies	>20 publications with ARG1 and Cardiovascular Diseases
Lung Neoplasms Antibodies	>20 publications with ARG1 and Lung Neoplasms
Asthma Antibodies	>19 publications with ARG1 and Asthma

Organs/Tissues associated with ARG1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>151 publications with ARG1 and Blood
Muscle Antibodies	>99 publications with ARG1 and Muscle
Brain Antibodies	>69 publications with ARG1 and Brain
Lung Antibodies	>65 publications with ARG1 and Lung
Liver Antibodies	>43 publications with ARG1 and Liver
Eye Antibodies	>25 publications with ARG1 and Eye
Bone Marrow Antibodies	>19 publications with ARG1 and Bone Marrow
Spleen Antibodies	>15 publications with ARG1 and Spleen
Skin Antibodies	>12 publications with ARG1 and Skin
Uterus Antibodies	>9 publications with ARG1 and Uterus