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Agrin (AGRN) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201193

Agrin, also known as AGRN, is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during… More details

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Product Overview

Online Datasheet in PDF
Product Name
Agrin (Agrn), Recombinant Protein
Full Product Name
Agrin
Product Gene Name
Agrn recombinant protein
Product Synonym Names
AGRN; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; AGR
Sequence
SCYNSPL GCCSDGKTPS LDSEGSNCPA TKAFQGVLEL EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFWSVR LRELGPGKLV RAIVDVHFDP TTAFQASDVG QALLRQIQVS RPWALAVRRP LQEHVRFLDF DWFPTFFTGA ATGTTAAMAT ARATTVSRLP ASSVTPRVYP SHTSRPVGRT TAPPTTRRPP TTATNMDRPR TPGHQQPSKS CDSQPCLHGG TCQDQDSGKG FTCSCTAGRG GSVCEKVQPP SMPAFKGHSF LAFPTLRAYH TLRLALEFRA LETEGLLLYN GNARGKDFLA LALLDGRVQF RFDTGSGPAV LTSLVPVEPG RWHRLELSRH WRQGTLSVDG ETPVVGESPS GTDGLNLDTN LYVGGIPEEQ VAMVLDRTSV GVGLKGCIRM LDINNQQLEL SDWQRAAVQS SGVGECGDHP CLPNPCHGGA LCQALEAGMF LCQCPPGRFG PTCADEKSPC QPNPCHGAAP CRVLSSGGAK CECPLGRSGT FCQTVLETAG SRPFLADFNG FSYLELKGLH TFERDLGEKM ALEMVFLARG PSGLLLYNGQ KTDGKGDFVS LALHNRHLEF CYDLGKGAAV IRSKEPIALG TWVRVFLERN GRKGALQVGD GPRVLGESPK SRKVPHTMLN LKEPLYIGGA PDFSKLARGA AVSSGFSGVI QLVSLRGHQL LTQEHVLRAV DVSPFADHPC TQALGNPCLN GGSCVPREAT YECLCPGGFS GLHCEKGLVE
Host
Baculovirus
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity/Purification
>90% by SDS - PAGE
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Molecular Weight
82.5kDa (766aa)
Sequence Positions
997-1753aa
3D Structure
ModBase 3D Structure for P25304
Antigen Species
Rat
Tag
His-Tag
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Predicted N-Terminal
Ala

Product Categories/Family for Agrn recombinant protein

Neuroscience
Applications Tested/Suitable for Agrn recombinant protein
SDS-PAGE

SDS-Page of Agrn recombinant protein

3ug by SDS PAGE under reducing condition and visualized by coomassie blue stain
AGRN Recombinant Protein – Cat# MBS201193

Product References and Citations for Agrn recombinant protein

Neuron., et al,(1991) Rupp F. 6(5):811-823.; Cell., et al,(1996) Glass DJ. 17; 85(4):513-523.

Other Notes

Small volumes of Agrn recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Agrn purchase

MBS201193 is a recombinant protein targeting Agrn. Also known as Agrin. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Agrn. It may not necessarily be applicable to this product.

NCBI GI #
28461145
NCBI GeneID
25592
NCBI Accession #
NP_786930
UniProt Primary Accession #
P25304
UniProt Related Accession #
P25304
GenBank Nucleotide #
NP_786930.1
NCBI Official Full Name
agrin
NCBI Official Synonym Full Names
agrin
NCBI Official Symbol
Agrn
NCBI Official Synonym Symbols
AGR
UniProt Protein Name
Agrin
Protein Family
Agrin
UniProt Gene Name
Agrn
UniProt Synonym Gene Names
Agrin; C90; C22
UniProt Entry Name
AGRIN_RAT
NCBI Protein Information
agrin

NCBI Summary for Agrn

may play a role in synapse development and regeneration [RGD, Feb 2006]

UniProt Comments for Agrn

AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.

Protein type: Cell adhesion; Apoptosis; Cell development/differentiation; Extracellular matrix; Cell surface; Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; plasma membrane; integral to membrane; basement membrane; synapse; basal lamina; cell junction

Molecular Function: heparan sulfate proteoglycan binding; protein binding; transforming growth factor beta binding; acetylcholine receptor regulator activity; calcium ion binding; sialic acid binding

Biological Process: positive regulation of filopodium formation; regulation of protein amino acid phosphorylation; positive regulation of protein binding; neurotransmitter receptor metabolic process; positive regulation of synaptic growth at neuromuscular junction; receptor clustering; synaptic transmission; synaptogenesis; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; protein heterotetramerization; regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; positive regulation of protein amino acid phosphorylation; neuromuscular junction development
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Research Articles on Agrn

1. Study in rat sepsis model demonstrates that agrin/MuSK pathway is associated with neuromuscular dysfunction and reexpression of gamma- and alpha7-nAChR caused by sepsis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Agrn recombinant protein

 Reference Product  PubMed Publications
 Ncam1 recombinant protein  >1 publications with Agrn and Ncam1

Pathways associated with Agrn recombinant protein

 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Defective B3GAT3 Causes JDSSDHD Pathway antibodies  Defective B3GAT3 Causes JDSSDHD Pathway Diagram
 Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway antibodies  Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway Diagram
 Defective B4GALT7 Causes EDS, Progeroid Type Pathway antibodies  Defective B4GALT7 Causes EDS, Progeroid Type Pathway Diagram
 Defective CHST14 Causes EDS, Musculocontractural Type Pathway antibodies  Defective CHST14 Causes EDS, Musculocontractural Type Pathway Diagram
 Defective CHST3 Causes SEDCJD Pathway antibodies  Defective CHST3 Causes SEDCJD Pathway Diagram
 Defective CHST6 Causes MCDC1 Pathway antibodies  Defective CHST6 Causes MCDC1 Pathway Diagram
 Defective CHSY1 Causes TPBS Pathway antibodies  Defective CHSY1 Causes TPBS Pathway Diagram

Diseases associated with Agrn recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >10 publications with Agrn and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with Agrn and Brain Diseases
 Congenital Abnormalities Antibodies  >1 publications with Agrn and Congenital Abnormalities
 Cognition Disorders Antibodies  >1 publications with Agrn and Cognition Disorders
 Cell Transformation, Neoplastic Antibodies  >1 publications with Agrn and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >1 publications with Agrn and Disease Models, Animal
 Death Antibodies  >1 publications with Agrn and Death
 Kidney Diseases Antibodies  >1 publications with Agrn and Kidney Diseases

Organs/Tissues associated with Agrn recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with Agrn and Brain
 Embryonic Tissue Antibodies  >4 publications with Agrn and Embryonic Tissue
 Heart Antibodies  >2 publications with Agrn and Heart
 Kidney Antibodies  >1 publications with Agrn and Kidney
 Dorsal Root Ganglion Antibodies  >1 publications with Agrn and Dorsal Root Ganglion
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