• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IL-33 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201198

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the… More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-33, Monoclonal Antibody
Full Product Name
IL-33
Product Gene Name
anti-IL-33 antibody
Product Synonym Names
Interleukin 33; DVS27; NF-HEV; NKHEV; C9orf26; IL-33; Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27 related protein; IL 1F11; IL 33; IL1F11; Interleukin 1 family member 11; INTERLEuKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2; il33
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
4E9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
608678
3D Structure
ModBase 3D Structure for O95760
Antigen Species
Human
Immunogen
Recombinant human IL-33 (112-270aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-IL-33 antibody

Cardiovascular; Immunology
Applications Tested/Suitable for anti-IL-33 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-IL-33 antibody
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity.

Western Blot (WB) of anti-IL-33 antibody

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate
Mouse Anti-IL-33 Monoclonal Antibody – Cat# MBS201198

Flow Cytometry (FC/FACS) of anti-IL-33 antibody

Flow cytometry analysis of IL-33 in Jurkat cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-IL-33 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS201198

Product References and Citations for anti-IL-33 antibody

Brint Ek, et al., (2005) J Biol Chem. 20:277(51).; Schmitz, et al., (2005) immunity. 23:479.; Baekkevold ES, et al., (2003) Am.J.Pathol. 163(1):69-90.

Other Notes

Small volumes of anti-IL-33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-33 purchase

MBS201198 is an antibody targeting IL-33. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-33. It may not necessarily be applicable to this product.

NCBI GI #
15559209
NCBI GeneID
90865
NCBI Accession #
NP_254274
UniProt Primary Accession #
O95760
UniProt Related Accession #
O95760
GenBank Nucleotide #
NP_254274.1
NCBI Official Full Name
interleukin-33 isoform a
NCBI Official Synonym Full Names
interleukin 33
NCBI Official Symbol
IL33
NCBI Official Synonym Symbols
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
UniProt Protein Name
Interleukin-33
UniProt Synonym Protein Names
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules
UniProt Gene Name
IL33
UniProt Synonym Gene Names
C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
UniProt Entry Name
IL33_HUMAN
NCBI Protein Information
interleukin-33

NCBI Summary for IL-33

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Show more

UniProt Comments for IL-33

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation
Show more

Research Articles on IL-33

1. These results demonstrate that type 2 inflammation and indirect AHR in asthma are related to a shift in mast cell infiltration to the airway epithelium, and that mast cells cooperate with epithelial cells through IL-33 signaling to regulate type 2 inflammation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL-33 antibody

 Reference Product  PubMed Publications
 IL1RL1 antibody  >157 publications with IL-33 and IL1RL1
 TSLP antibody  >19 publications with IL-33 and TSLP
 CASP1 antibody  >17 publications with IL-33 and CASP1
 IL18 antibody  >16 publications with IL-33 and IL18
 IL25 antibody  >14 publications with IL-33 and IL25
 IL18R1 antibody  >7 publications with IL-33 and IL18R1
 VEGFA antibody  >3 publications with IL-33 and VEGFA
 IL1B antibody  >2 publications with IL-33 and IL1B
 CSF2 antibody  >1 publications with IL-33 and CSF2

Pathways associated with anti-IL-33 antibody

 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram

Diseases associated with anti-IL-33 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >186 publications with IL-33 and Inflammation
 Asthma Antibodies  >86 publications with IL-33 and Asthma
 Hypersensitivity, Immediate Antibodies  >82 publications with IL-33 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >81 publications with IL-33 and Lung Diseases
 Necrosis Antibodies  >55 publications with IL-33 and Necrosis
 Cardiovascular Diseases Antibodies  >50 publications with IL-33 and Cardiovascular Diseases
 Heart Diseases Antibodies  >30 publications with IL-33 and Heart Diseases
 Fibrosis Antibodies  >20 publications with IL-33 and Fibrosis
 Dermatitis, Atopic Antibodies  >18 publications with IL-33 and Dermatitis, Atopic
 Disease Models, Animal Antibodies  >18 publications with IL-33 and Disease Models, Animal

Organs/Tissues associated with anti-IL-33 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >53 publications with IL-33 and Lung
 Skin Antibodies  >42 publications with IL-33 and Skin
 Heart Antibodies  >42 publications with IL-33 and Heart
 Vascular Antibodies  >33 publications with IL-33 and Vascular
 Muscle Antibodies  >19 publications with IL-33 and Muscle
 Intestine Antibodies  >17 publications with IL-33 and Intestine
 Liver Antibodies  >16 publications with IL-33 and Liver
 Brain Antibodies  >12 publications with IL-33 and Brain
 Eye Antibodies  >9 publications with IL-33 and Eye
 Connective Tissue Antibodies  >7 publications with IL-33 and Connective Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000