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GRP78 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201207

Binding immunoglobulin protein (BIP) also known as 78kDa glucose-regulated protein (GRP-78) or heatshock 70kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmicreticulum (ER) that binds newly synthesized proteins as they… More details

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Product Overview

Online Datasheet in PDF
Product Name
GRP78/HSPA5, Monoclonal Antibody
Full Product Name
GRP78/HSPA5
Product Gene Name
anti-GRP78 antibody
Product Synonym Gene Name
HSPA5
Product Synonym Names
Binding immunoglobulin protein; HSPA5; heat shock 70kDa protein 5; GRP78; glucose-regulated protein 78kD; MIF2
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT3D2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
138120
3D Structure
ModBase 3D Structure for P11021
Antigen Species
Human
Immunogen
Recombinant human BIP (20-650aa) purified from Baculo virus
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-GRP78 antibody

Cancer; Lysates; Metabolism; Neuroscience; Other; Signal Transduction; Stem cell; Tags & Cell Markers
Applications Tested/Suitable for anti-GRP78 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GRP78 antibody
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity.

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-GRP78 antibody

ICC/IF analysis of GRP78/HSPA5 in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-GRP78 Monoclonal Antibody – Cat# MBS201207

Western Blot (WB) of anti-GRP78 antibody

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP78/HSPA5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: U87MG cell lysate Lane 3.: Lncap cell lysate
Mouse Anti-GRP78 Monoclonal Antibody, Western Blot (WB) – Cat# MBS201207

Product References and Citations for anti-GRP78 antibody

Ting. J., et al. (1988) DNA 7(4): 275-86.; Hendershot. L.M., et al. (1994) Genomics 20(2): 281-284.

Other Notes

Small volumes of anti-GRP78 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRP78 purchase

MBS201207 is an antibody targeting GRP78. Also known as GRP78/HSPA5. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GRP78. It may not necessarily be applicable to this product.

NCBI GI #
16507237
NCBI GeneID
3309
NCBI Accession #
NP_005338
UniProt Primary Accession #
P11021
UniProt Related Accession #
P11021
GenBank Nucleotide #
NP_005338.1
NCBI Official Full Name
endoplasmic reticulum chaperone BiP
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 5
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Synonym Protein Names
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein
Protein Family
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
NCBI Protein Information
endoplasmic reticulum chaperone BiP

NCBI Summary for GRP78

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
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UniProt Comments for GRP78

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; focal adhesion; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; unfolded protein binding; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; platelet degranulation; substantia nigra development; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on GRP78

1. Inflammatory cytokines induce a self-destructive pro-apoptotic feedback loop through the secretion and membrane translocation of GRP78.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GRP78 antibody

 Reference Product  PubMed Publications
 SIL1 antibody  >4 publications with GRP78 and SIL1
 ERN1 antibody  >4 publications with GRP78 and ERN1
 EIF2AK3 antibody  >2 publications with GRP78 and EIF2AK3
 ERP29 antibody  >1 publications with GRP78 and ERP29
 CASP7 antibody  >1 publications with GRP78 and CASP7

Pathways associated with anti-GRP78 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Chaperone Genes By ATF6-alpha Pathway antibodies  Activation Of Chaperone Genes By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By ATF6-alpha Pathway antibodies  Activation Of Chaperones By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By IRE1alpha Pathway antibodies  Activation Of Chaperones By IRE1alpha Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-GRP78 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >49 publications with GRP78 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >47 publications with GRP78 and Nervous System Diseases
 Disease Models, Animal Antibodies  >42 publications with GRP78 and Disease Models, Animal
 Brain Diseases Antibodies  >40 publications with GRP78 and Brain Diseases
 Drug Toxicity Antibodies  >34 publications with GRP78 and Drug Toxicity
 Neoplasms Antibodies  >30 publications with GRP78 and Neoplasms
 Inflammation Antibodies  >20 publications with GRP78 and Inflammation
 Heart Diseases Antibodies  >18 publications with GRP78 and Heart Diseases
 Liver Diseases Antibodies  >16 publications with GRP78 and Liver Diseases
 Necrosis Antibodies  >15 publications with GRP78 and Necrosis

Organs/Tissues associated with anti-GRP78 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >61 publications with GRP78 and Brain
 Muscle Antibodies  >40 publications with GRP78 and Muscle
 Blood Antibodies  >32 publications with GRP78 and Blood
 Heart Antibodies  >31 publications with GRP78 and Heart
 Liver Antibodies  >29 publications with GRP78 and Liver
 Kidney Antibodies  >26 publications with GRP78 and Kidney
 Vascular Antibodies  >17 publications with GRP78 and Vascular
 Nerve Antibodies  >16 publications with GRP78 and Nerve
 Pancreas Antibodies  >13 publications with GRP78 and Pancreas
 Testis Antibodies  >11 publications with GRP78 and Testis
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