• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MEMO1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201209

MEMO1 (Mediator of ErbB2-driven cell motility 1) is a 297 amino acid protein. It is thought to relax extracellular chemotactic signals that are targeted at the microtubule cytoskeleton, thereby controlling cell migration. Additionally, MEMO1 is required for… More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MEMO1, Monoclonal Antibody
Full Product Name
MEMO1
Product Gene Name
anti-MEMO1 antibody
Product Synonym Names
Protein MEMO1; C2orf4; CGI-27; MEMO; NS5ATP7
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT1E9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
611786
3D Structure
ModBase 3D Structure for Q9Y316
Antigen Species
Human
Immunogen
Recombinant human MEMO1 (1-297aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-MEMO1 antibody

Signal Transduction
Applications Tested/Suitable for anti-MEMO1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-MEMO1 antibody
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity.

Western Blot (WB) of anti-MEMO1 antibody

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: U87MG cell lysate
Mouse Anti-MEMO1 Monoclonal Antibody – Cat# MBS201209

Flow Cytometry (FC/FACS) of anti-MEMO1 antibody

Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-MEMO1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS201209

Product References and Citations for anti-MEMO1 antibody

Marone R., et al. (2004) Nat Cell Biol 6(6):515-522; Meira M., et al. (2009) J Cell Sci 122:787-797; Zaoui K., et al. (2010) Proc Natl Acad Sci USA 107(43):18517-18522

Other Notes

Small volumes of anti-MEMO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEMO1 purchase

MBS201209 is an antibody targeting MEMO1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MEMO1. It may not necessarily be applicable to this product.

NCBI GI #
7705720
NCBI GeneID
51072
NCBI Accession #
NP_057039
UniProt Primary Accession #
Q9Y316
UniProt Related Accession #
Q9Y316
GenBank Nucleotide #
NP_057039.1
NCBI Official Full Name
protein MEMO1 isoform 1
NCBI Official Synonym Full Names
mediator of cell motility 1
NCBI Official Symbol
MEMO1
NCBI Official Synonym Symbols
MEMO; C2orf4; CGI-27; NS5ATP7
UniProt Protein Name
Protein MEMO1
UniProt Synonym Protein Names
C21orf19-like protein; Hepatitis C virus NS5A-transactivated protein 7; HCV NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Mediator of cell motility 1; Memo-1
Protein Family
Protein
UniProt Gene Name
MEMO1
UniProt Synonym Gene Names
C2orf4; MEMO; NS5ATP7; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; Memo-1
UniProt Entry Name
MEMO1_HUMAN
NCBI Protein Information
protein MEMO1

UniProt Comments for MEMO1

MEMO1: a highly conserved protein that controls cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Regulates directionality during HER2-dependent cell migration. Controls microtubules, the actin network, and adhesion site formation in migrating cells via the MEMO1-RHOA-DIAPH1 signaling pathway. Homologous to class III nonheme iron-dependent dioxygenases. No metal-binding or enzymatic activity has been detected. Binds HER2 phosphorylated at Y1222 via its vestigial enzymatic active site. Binds to and influences insulin-like growth factor-I (IRS1) receptor-dependent signaling events in breast carcinogenesis. Influences the epithelial-mesenchymal transition (EMT) via the IRS1/PI3K/Akt/Snail1 pathway. Interacts with cofilin, and influences PLCg1 and cofilin activities. Increased expression is associated with cancer aggressiveness. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Two isoform of the human protein are produced by alternatuve splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleus; cytosol

Molecular Function: protein binding

Biological Process: regulation of microtubule-based process
Show more

Research Articles on MEMO1

1. Memo1 is a mediator of radial glial cell scaffold tiling, necessary to generate and organize neurons into functional ensembles in the developing cerebral cortex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MEMO1 antibody

 Reference Product  PubMed Publications
 ERBB2 antibody  >9 publications with MEMO1 and ERBB2

Organs/Tissues associated with anti-MEMO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with MEMO1 and Blood
 Mammary Gland Antibodies  >1 publications with MEMO1 and Mammary Gland
 Lung Antibodies  >1 publications with MEMO1 and Lung
 Eye Antibodies  >1 publications with MEMO1 and Eye
 Skin Antibodies  >1 publications with MEMO1 and Skin
 Brain Antibodies  >1 publications with MEMO1 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000