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Coactosin-Like Protein 1/COTL1 (COTL1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201218

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal proteinthat belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified inslime mold and is found at highest levels in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Coactosin-like Protein 1/COTL1, Monoclonal Antibody
Full Product Name
Coactosin-like Protein 1/COTL1
Product Gene Name
Product Synonym Names
Coactosin-like protein; CLP; FLJ43657; MGC19733
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT1D6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
606748
3D Structure
ModBase 3D Structure for Q14019
Antigen Species
Human
Immunogen
Recombinant human COTL1 (1-142aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-COTL1 antibody

Applications Tested/Suitable for anti-COTL1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-COTL1 antibody
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity.

Western Blot (WB) of anti-COTL1 antibody

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate
Mouse Anti-COTL1 Monoclonal Antibody – Cat# MBS201218

Flow Cytometry (FC/FACS) of anti-COTL1 antibody

Flow cytometry analysis of Coactosin-like Protein 1/COTL1 in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-COTL1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS201218

Product References and Citations for anti-COTL1 antibody

Provost. P., et al. (2001) Biochem J 359(1): 255-263.; Dai. H., et al. (2006) Biochim Biophys Acta 1764(11): 1688-1700.; Esser. J., et al. (2010) Biochem J 425(1): 265-274.

Other Notes

Small volumes of anti-COTL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COTL1 purchase

MBS201218 is an antibody targeting COTL1. Also known as Coactosin-like Protein 1/COTL1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for COTL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
coactosin-like protein
NCBI Official Synonym Full Names
coactosin like F-actin binding protein 1
NCBI Official Symbol
COTL1
NCBI Official Synonym Symbols
CLP
UniProt Protein Name
Coactosin-like protein
UniProt Gene Name
COTL1
UniProt Synonym Gene Names
CLP
UniProt Entry Name
COTL1_HUMAN
NCBI Protein Information
coactosin-like protein

NCBI Summary for COTL1

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008]
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UniProt Comments for COTL1

COTL1: Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Belongs to the actin-binding proteins ADF family. Coactosin subfamily.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nuclear membrane; cytoskeleton; cytoplasm

Molecular Function: protein binding; enzyme binding; actin binding

Biological Process: defense response to fungus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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