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PARP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201220

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable ofcatalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to thatof poly (ADP-ribosyl) transferase, but… More details

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Product Overview

Online Datasheet in PDF
Product Name
PARP2, Monoclonal Antibody
Full Product Name
PARP2
Product Gene Name
anti-PARP2 antibody
Product Synonym Names
Poly (ADP-ribose) polymerase 2; ADPRT2; ADPRTL2; ADPRTL3; ARTD2; pADPRT-2; PARP-2
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT29G4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
Protein-A affinity chromatography
Concentration
1mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
OMIM
607725
3D Structure
ModBase 3D Structure for Q9UGN5
Immunogen
Recombinant human PARP2 (233-583aa) purified from E. coli
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-PARP2 antibody

Epigenetics and Nuclear Signaling
Applications Tested/Suitable for anti-PARP2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-PARP2 antibody
The antibody has been tested by ELISA, Western blot, ICC/IF and FC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

SDS-PAGE of anti-PARP2 antibody

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: SW480 cell lysate
Mouse Anti-PARP2 Monoclonal Antibody – Cat# MBS201220

SDS-PAGE of anti-PARP2 antibody

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Mouse Anti-PARP2 Monoclonal Antibody, SDS-PAGE – Cat# MBS201220

SDS-PAGE of anti-PARP2 antibody

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: NIH3T3 cell lysate
Lane 2.: Raji cell lysate
Mouse Anti-PARP2 Monoclonal Antibody, SDS-PAGE – Cat# MBS201220

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-PARP2 antibody

ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with MBS201220 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-PARP2 Monoclonal Antibody, Immunocytochemistry (ICC)/Immunofluorescence – Cat# MBS201220

Flow Cytometry (FC) of anti-PARP2 antibody

Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with MBS201220 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-PARP2 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS201220

Product References and Citations for anti-PARP2 antibody

Ame JC, Rolli V, Schreiber V et al. (1999). J. Biol. Chem. 274 (25): 17860-8.; Schreiber V, Ame JC, Dolle P et al. (2002). J. Biol. Chem. 277 (25): 23028-36.; Maeda Y, Hunter TC, Loudy DE et al. (2006). J. Biol. Chem. 281 (14): 9600-6.

Other Notes

Small volumes of anti-PARP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PARP2 purchase

MBS201220 is an antibody targeting PARP2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PARP2. It may not necessarily be applicable to this product.

NCBI GI #
110825961
NCBI GeneID
10038
NCBI Accession #
NP_005475
UniProt Primary Accession #
Q9UGN5
UniProt Related Accession #
Q9UGN5
GenBank Nucleotide #
NP_005475.2
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase 2
NCBI Official Symbol
PARP2
NCBI Official Synonym Symbols
ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
UniProt Protein Name
Poly [ADP-ribose] polymerase 2
UniProt Synonym Protein Names
ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; NAD(+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2
Protein Family
Poly [ADP-ribose] polymerase
UniProt Gene Name
PARP2
UniProt Synonym Gene Names
ADPRT2; ADPRTL2; PARP-2; hPARP-2; ARTD2; ADPRT-2; pADPRT-2
UniProt Entry Name
PARP2_HUMAN
NCBI Protein Information
poly [ADP-ribose] polymerase 2

NCBI Summary for PARP2

This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
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UniProt Comments for PARP2

PARP2: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Transferase; EC 2.4.2.30

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; base-excision repair; DNA repair
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Research Articles on PARP2

1. HPF1 completes the PARP active site for DNA damage-induced ADP-ribosylation; as HPF1 forms a joint active site with PARP1 or PARP2, our data implicate HPF1 as an important determinant of the response to clinical PARP inhibitors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PARP2 antibody

 Reference Product  PubMed Publications
 PARP1 antibody  >85 publications with PARP2 and PARP1
 SIRT1 antibody  >4 publications with PARP2 and SIRT1
 XRCC1 antibody  >2 publications with PARP2 and XRCC1

Pathways associated with anti-PARP2 antibody

 Products by Pathway  Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram

Diseases associated with anti-PARP2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with PARP2 and Nervous System Diseases
 Necrosis Antibodies  >8 publications with PARP2 and Necrosis
 Breast Neoplasms Antibodies  >7 publications with PARP2 and Breast Neoplasms
 Inflammation Antibodies  >6 publications with PARP2 and Inflammation
 Cardiovascular Diseases Antibodies  >6 publications with PARP2 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >3 publications with PARP2 and Neoplasms, Experimental
 Anemia Antibodies  >3 publications with PARP2 and Anemia
 Adenocarcinoma Antibodies  >2 publications with PARP2 and Adenocarcinoma
 Infertility, Male Antibodies  >2 publications with PARP2 and Infertility, Male
 Ovarian Neoplasms Antibodies  >2 publications with PARP2 and Ovarian Neoplasms

Organs/Tissues associated with anti-PARP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with PARP2 and Blood
 Brain Antibodies  >9 publications with PARP2 and Brain
 Lung Antibodies  >7 publications with PARP2 and Lung
 Kidney Antibodies  >4 publications with PARP2 and Kidney
 Heart Antibodies  >3 publications with PARP2 and Heart
 Liver Antibodies  >3 publications with PARP2 and Liver
 Skin Antibodies  >3 publications with PARP2 and Skin
 Testis Antibodies  >2 publications with PARP2 and Testis
 Thymus Antibodies  >2 publications with PARP2 and Thymus
 Intestine Antibodies  >2 publications with PARP2 and Intestine
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