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ACAT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201225

ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ACAT1, Monoclonal Antibody
Full Product Name
ACAT1
Product Gene Name
anti-ACAT1 antibody
Product Synonym Names
acetyl-CoA acetyltransferase 1; ACAT; MAT; T2; THIL
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
2C5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
203750
3D Structure
ModBase 3D Structure for P24752
Antigen Species
Human
Immunogen
Recombinant human ACAT1 (34-427aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-ACAT1 antibody

Cancer; Cardiovascular; Metabolism; Signal Transduction; Tags & Cell Markers
Applications Tested/Suitable for anti-ACAT1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ACAT1 antibody
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity.

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-ACAT1 antibody

ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-ACAT1 Monoclonal Antibody – Cat# MBS201225

Western Blot (WB) of anti-ACAT1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Hep3B cell lysate Lane 4.: 293T cell lysate Lane 5.: A431 cell lysate
Mouse Anti-ACAT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS201225

Product References and Citations for anti-ACAT1 antibody

Antonenkov, V.D., et al. (2000) Eur J Biochem 267: 2981-2990.; Korman, S.H. (2006) Mol Genet Metab 89: 289-299.; Yamaguchi, S., et al. (1988) J Clin Invest 81(3): 813-817.

Other Notes

Small volumes of anti-ACAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACAT1 purchase

MBS201225 is an antibody targeting ACAT1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.

NCBI GI #
4557237
NCBI GeneID
38
NCBI Accession #
NP_000010
UniProt Primary Accession #
P24752
UniProt Related Accession #
P24752
GenBank Nucleotide #
NP_000010.1
NCBI Official Full Name
acetyl-CoA acetyltransferase, mitochondrial
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 1
NCBI Official Symbol
ACAT1
NCBI Official Synonym Symbols
T2; MAT; ACAT; THIL
UniProt Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
UniProt Synonym Protein Names
Acetoacetyl-CoA thiolase; T2
UniProt Gene Name
ACAT1
UniProt Synonym Gene Names
ACAT; MAT
UniProt Entry Name
THIL_HUMAN
NCBI Protein Information
acetyl-CoA acetyltransferase, mitochondrial

NCBI Summary for ACAT1

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
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UniProt Comments for ACAT1

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Mitochondrial; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - lysine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; branched chain family amino acid catabolic process; cellular lipid metabolic process; brain development; ketone body metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria
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Research Articles on ACAT1

1. Silencing ACAT1 attenuates Abeta-induced cytotoxicity and cell apoptosis in SH-SY5Y cells, which may due to the synergistic effect of ACAT1 and COX2 through PKC/ERK pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACAT1 antibody

 Products by Pathway  Pathway Diagram
 Branched-chain Amino Acid Catabolism Pathway antibodies  Branched-chain Amino Acid Catabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram

Diseases associated with anti-ACAT1 antibody

 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >64 publications with ACAT1 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >46 publications with ACAT1 and Cardiovascular Diseases
 Hypercholesterolemia Antibodies  >16 publications with ACAT1 and Hypercholesterolemia
 Neoplasms Antibodies  >15 publications with ACAT1 and Neoplasms
 Dyslipidemias Antibodies  >15 publications with ACAT1 and Dyslipidemias
 Hyperlipidemias Antibodies  >14 publications with ACAT1 and Hyperlipidemias
 Nervous System Diseases Antibodies  >13 publications with ACAT1 and Nervous System Diseases
 Fatty Liver Antibodies  >11 publications with ACAT1 and Fatty Liver
 Liver Diseases Antibodies  >9 publications with ACAT1 and Liver Diseases
 Hypertension Antibodies  >8 publications with ACAT1 and Hypertension

Organs/Tissues associated with anti-ACAT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >44 publications with ACAT1 and Blood
 Liver Antibodies  >40 publications with ACAT1 and Liver
 Intestine Antibodies  >18 publications with ACAT1 and Intestine
 Brain Antibodies  >9 publications with ACAT1 and Brain
 Muscle Antibodies  >8 publications with ACAT1 and Muscle
 Ovary Antibodies  >7 publications with ACAT1 and Ovary
 Bone Antibodies  >6 publications with ACAT1 and Bone
 Heart Antibodies  >5 publications with ACAT1 and Heart
 Kidney Antibodies  >5 publications with ACAT1 and Kidney
 Adipose Tissue Antibodies  >4 publications with ACAT1 and Adipose Tissue
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