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Phospholipase A2, Group IIA (PLA2G2A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2016532
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2016532 is an antibody targeting PLA2G2A. Also known as Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A). Reactive in Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phospholipase A2, Group IIA (PLA2G2A), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SLL EFGQMI LFKTGKRADV SYGFYGCHCG VGGRGSPKDA TDWCCVTHDC CYNRLEKRGC GTKFLTYKFS YRGGQISCST NQDSCRKQLC QCDKAAAECF ARNKKSYSLK YQFYPNKFCK
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against PLA2G2A. It has been selected for its ability to recognize PLA2G2A in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: PLA2G2A (MBS2016532)
Immunogen: PLA2G2A (MBS2018441)
Matching Pairs
Unconjugated Antibody: PLA2G2A (MBS2016532)
APC-CY7 Conjugated Antibody: PLA2G2A (MBS2073089)
Molecular Weight
16,294 Da
3D Structure
ModBase 3D Structure for P14423
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant PLA2G2A (Ser22~Cys146) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073091
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PLA2G2A antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-PLA2G2A antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PLA2G2A antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-PLA2G2A Polyclonal Antibody – Cat# MBS2016532

Immunohistochemistry (IHC) of anti-PLA2G2A antibody

DAB staining on fromalin fixed paraffin- embedded testis tissue)
Rabbit Anti-PLA2G2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2016532

Immunohistochemistry (IHC) of anti-PLA2G2A antibody

DAB staining on IHC-P; Samples: Rat Skin Tissue)
Rabbit Anti-PLA2G2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2016532

Other Notes

Small volumes of anti-PLA2G2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLA2G2A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2 group IIA
NCBI Official Symbol
Pla2g2a
NCBI Official Synonym Symbols
sPLA2
UniProt Protein Name
Phospholipase A2, membrane associated
UniProt Synonym Protein Names
GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A
Protein Family
UniProt Gene Name
Pla2g2a
NCBI Protein Information
phospholipase A2, membrane associated

NCBI Summary for PLA2G2A

hydrolyzes acyl group at the sn-2 position of phospholipids, forming non-esterified fatty acids and lysophospholipids; plays a critical role in inflammation; induces proliferation of smooth muscle cells [RGD, Feb 2006]

UniProt Comments for PLA2G2A

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:3356705). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Independent of its catalytic activity, activates integrins by binding to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and inducing integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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