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Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2016988
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2016988 is an antibody targeting PAFAH1B3. Also known as Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3). Reactive in Human. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.83mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: PAFAH1B3 (MBS2016988)
Immunogen: PAFAH1B3 (MBS2018339)
Matching Pairs
Unconjugated Antibody: PAFAH1B3 (MBS2016988)
APC-CY7 Conjugated Antibody: PAFAH1B3 (MBS2054728)
OMIM
D63391 mRNA
3D Structure
ModBase 3D Structure for Q15102
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Pig
Immunogen
Recombinant PAFAH1B3 (His28~Pro231 (Accession # Q15102)) expressed in E.coli (MBS2018339).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PAFAH1B3 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-PAFAH1B3 antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-PAFAH1B3 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-PAFAH1B3 Polyclonal Antibody – Cat# MBS2016988

Western Blot (WB) of anti-PAFAH1B3 antibody

Western Blot; Sample: Lane1: Jurkat cell lysate;
Lane2: Hela cell lysate Primary Ab: 1ug/ml Rabbit Anti-Human PAFAH1B3 Antibody
Second Ab: 0.2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-PAFAH1B3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2016988

Immunohistochemistry (IHC) of anti-PAFAH1B3 antibody

DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human PAFAH1B3 Antibody
Second Ab: 2?g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-PAFAH1B3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2016988

Other Notes

Small volumes of anti-PAFAH1B3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAFAH1B3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
platelet-activating factor acetylhydrolase IB subunit gamma
NCBI Official Synonym Full Names
platelet activating factor acetylhydrolase 1b catalytic subunit 3
NCBI Official Symbol
PAFAH1B3
NCBI Official Synonym Symbols
PAFAHG
UniProt Protein Name
Platelet-activating factor acetylhydrolase IB subunit gamma
UniProt Synonym Protein Names
PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAFAH subunit gamma
UniProt Gene Name
PAFAH1B3
UniProt Synonym Gene Names
PAFAHG; PAF-AH 29 kDa subunit; PAFAH subunit gamma
NCBI Protein Information
platelet-activating factor acetylhydrolase IB subunit gamma

NCBI Summary for PAFAH1B3

This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with cognitive disability, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
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UniProt Comments for PAFAH1B3

PAFAH1B3: Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Protein type: EC 3.1.1.47; Lipase; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cytoplasm; cytosol; membrane

Molecular Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; identical protein binding; phospholipase A2 activity; platelet-activating factor acetyltransferase activity; protein binding; protein heterodimerization activity

Biological Process: brain development; lipid catabolic process; lipid metabolic process; nervous system development; spermatogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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