Ribonuclease III, Nuclear (RNASEN) Antibody – Rabbit Polyclonal

This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]

DROSHA: a nuclear type-III RNase that, along with the DGCR8, executes the initial step of microRNA (miRNA) processing in the nucleus. Cleaves the local hairpin structures embedded in long primary microRNA transcripts (pri-miRNAs) into pre-miRNAs. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Interacts with Sp1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.26.3; Nucleolus; RNA processing; RNA-binding; Ribonuclease

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: focal adhesion; nucleolus; nucleoplasm; nucleus; plasma membrane; postsynaptic density

Molecular Function: DEAD/H-box RNA helicase binding; double-stranded RNA binding; lipopolysaccharide binding; metal ion binding; protein binding; protein homodimerization activity; ribonuclease III activity; RNA binding; SMAD binding

Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; pre-microRNA processing; primary microRNA processing; regulation of inflammatory response; regulation of regulatory T cell differentiation; ribosome biogenesis; RNA interference, production of siRNA; rRNA catabolic process