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Enolase (NSE) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025648
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2025648 is an antibody targeting NSE. Also known as Monoclonal Antibody to Enolase, Neuron Specific (NSE). Reactive in Homo sapiens (Human). Host: Mouse. More details

$200 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Enolase (NSE), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Enolase, Neuron Specific (NSE)
Product Gene Name
anti-NSE antibody
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C10
Host
Mouse
Species Reactivity
Homo sapiens (Human)
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: NSE Clone #C10 (MBS2025648)
Immunogen: NSE (MBS2010171)
Matching Pairs
Unconjugated Antibody: NSE Clone #C10 (MBS2025648)
APC-CY7 Conjugated Antibody: NSE Clone #C10 (MBS2043560)
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OMIM
X13120 mRNA
3D Structure
ModBase 3D Structure for P09104
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant NSE (Ser2~Arg285) expressed in E.coli (MBS2010171)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NSE antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunofluorescence (IF).
Application Notes for anti-NSE antibody
Western blotting: 0.01-2ug/mL
Immunohistochemistry: 5-30ug/mL
Immunofluorescence: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Immunohistochemistry Paraffin (IHC-P) of anti-NSE antibody

DAB staining on IHC-P;
Samples: Human Pancreatic cancer Tissue
Primary Ab: 30ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody – Cat# MBS2025648

Immunohistochemistry Paraffin (IHC-P) of anti-NSE antibody

DAB staining on IHC-P;
Samples: Human Glioma Tissue
Primary Ab: 30ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody, Immunohistochemistry Paraffin (IHC-P) – Cat# MBS2025648

Immunohistochemistry Paraffin (IHC-P) of anti-NSE antibody

DAB staining on IHC-P;
Samples: Human Cerebrum Tissue
Primary Ab: 30ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody, Immunohistochemistry Paraffin (IHC-P) – Cat# MBS2025648

Western Blot (WB) of anti-NSE antibody

Western Blot
Samples: Human Hela cell lysate
Primary Ab: 2ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025648

Western Blot (WB) of anti-NSE antibody

Western Blot
Samples: Human HepG2 cell lysate
Primary Ab: 2ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025648

Western Blot (WB) of anti-NSE antibody

Western Blot
Samples: Mouse Cerebrum lysate
Primary Ab: 2ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-NSE Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025648

Immunofluorescence (IF) of anti-NSE antibody

FITC staining on IF
Sample: Human HepG2 cell
Primary Ab: 20ug/ml Mouse Anti-Human NSE Antibody
Second Ab: 1ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086034)
Mouse Anti-NSE Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS2025648

Other Notes

Small volumes of anti-NSE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NSE. It may not necessarily be applicable to this product.

NCBI GI #
5803011
NCBI GeneID
2026
NCBI Accession #
NP_001966.1
UniProt Primary Accession #
P09104
UniProt Secondary Accession #
Q96J33; B7Z2X9
UniProt Related Accession #
P09104
GenBank Nucleotide #
NM_001975.2
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
Protein Family
Enolase
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
NCBI Protein Information
gamma-enolase

NCBI Summary for NSE

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

UniProt Comments for NSE

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular exosome; extracellular space; membrane; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis; glycolytic process
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Research Articles on NSE

1. serum level elevated in small cell lung cancer patients with shorter progression-free survival

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NSE antibody

 Reference Product  PubMed Publications
 HK1 antibody  >2 publications with NSE and HK1

Pathways associated with anti-NSE antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram

Diseases associated with anti-NSE antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with NSE and Neoplasms
 Nervous System Diseases Antibodies  >4 publications with NSE and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with NSE and Brain Diseases
 Adenocarcinoma Antibodies  >3 publications with NSE and Adenocarcinoma
 Lung Diseases Antibodies  >2 publications with NSE and Lung Diseases
 Inflammation Antibodies  >2 publications with NSE and Inflammation
 Kidney Diseases Antibodies  >2 publications with NSE and Kidney Diseases
 Hypertension Antibodies  >2 publications with NSE and Hypertension
 Uterine Cervical Neoplasms Antibodies  >2 publications with NSE and Uterine Cervical Neoplasms
 Prostatic Neoplasms Antibodies  >2 publications with NSE and Prostatic Neoplasms

Organs/Tissues associated with anti-NSE antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with NSE and Brain
 Blood Antibodies  >8 publications with NSE and Blood
 Muscle Antibodies  >7 publications with NSE and Muscle
 Intestine Antibodies  >4 publications with NSE and Intestine
 Lung Antibodies  >4 publications with NSE and Lung
 Skin Antibodies  >3 publications with NSE and Skin
 Kidney Antibodies  >3 publications with NSE and Kidney
 Bone Antibodies  >3 publications with NSE and Bone
 Liver Antibodies  >3 publications with NSE and Liver
 Embryonic Tissue Antibodies  >3 publications with NSE and Embryonic Tissue
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