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Arginase (ARG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025650
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MBS2025650 is an antibody targeting ARG. Also known as Monoclonal Antibody to Arginase (ARG). Reactive in Rattus norvegicus (Rat). Host: Mouse. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Arginase, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Arginase (ARG)
Product Gene Name
anti-ARG antibody
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
1-2#
Host
Mouse
Species Reactivity
Rattus norvegicus (Rat)
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Matching Pairs
Unconjugated Antibody: ARG Clone #1-2# (MBS2025650)
Immunogen: ARG (MBS2009782)
Matching Pairs
Unconjugated Antibody: ARG Clone #1-2# (MBS2025650)
APC-CY7 Conjugated Antibody: ARG Clone #1-2# (MBS2051737)
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3D Structure
ModBase 3D Structure for P07824
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Porcine
Immunogen
Recombinant ARG (Met1~Lys323) expressed in E.coli (MBS2009782)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-ARG antibody
Western Blot (WB); Immunohistochemistry (IHC)
Application Notes for anti-ARG antibody
Western blotting: 0.01-2ug/mL
Immunohistochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ARG antibody

Western Blot; Samples: Lane1: Rat Liver lysate; Lane2: Porcine Liver lysate; Primary Ab: 0.2ug/ml Mouse Anti-Rat Arg Antibody Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-ARG Monoclonal Antibody – Cat# MBS2025650

Immunohistochemistry (IHC) of anti-ARG antibody

DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat ARG Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-ARG Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025650

Western Blot (WB) of anti-ARG antibody

Figure.Western Blot Lane1: Rat Serum Lane2: Rat Liver Tissue Primary Ab: 2ug/mL Rabbit Anti-Rat Arg Ab Second Ab: 1:5000 Dilution of HRP- Linked Rabbit Anti-Mouse IgG Ab (Catalog: MBS2086032)
Mouse Anti-ARG Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025650

Other Notes

Small volumes of anti-ARG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARG. It may not necessarily be applicable to this product.

NCBI GI #
166091458
NCBI GeneID
29221
NCBI Accession #
NP_058830.2
UniProt Primary Accession #
P07824
UniProt Secondary Accession #
Q5BK93
UniProt Related Accession #
P07824
GenBank Nucleotide #
NM_017134.3
NCBI Official Full Name
arginase-1
NCBI Official Synonym Full Names
arginase 1
NCBI Official Symbol
Arg1
UniProt Protein Name
Arginase-1
UniProt Synonym Protein Names
Liver-type arginase; Type I arginase
Protein Family
Arginase
UniProt Gene Name
Arg1
NCBI Protein Information
arginase-1

NCBI Summary for ARG

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

UniProt Comments for ARG

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cell soma; cytoplasm; extracellular space; mitochondrial outer membrane; neuron projection; nucleus

Molecular Function: arginase activity; manganese ion binding

Biological Process: aging; arginine catabolic process to ornithine; arginine metabolic process; collagen biosynthetic process; defense response to protozoan; female pregnancy; liver development; lung development; mammary gland involution; maternal process involved in female pregnancy; negative regulation of activated T cell proliferation; negative regulation of T cell proliferation; positive regulation of endothelial cell proliferation; response to amine; response to amino acid; response to axon injury; response to cadmium ion; response to drug; response to herbicide; response to lipopolysaccharide; response to manganese ion; response to methylmercury; response to peptide hormone; response to selenium ion; response to steroid hormone; response to vitamin A; response to vitamin E; response to wounding; response to zinc ion; urea cycle
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Research Articles on ARG

1. Tissue-specific up-regulation of arginase I and II induced by p38 MAPK mediates endothelial dysfunction in type 1 diabetes mellitus

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARG antibody

 Reference Product  PubMed Publications
 Nos2 antibody  >75 publications with ARG and Nos2
 Nos3 antibody  >14 publications with ARG and Nos3
 Asl antibody  >7 publications with ARG and Asl
 Nos1 antibody  >6 publications with ARG and Nos1
 Oat antibody  >6 publications with ARG and Oat
 Otc antibody  >6 publications with ARG and Otc

Pathways associated with anti-ARG antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-ARG antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >160 publications with ARG and Inflammation
 Disease Models, Animal Antibodies  >90 publications with ARG and Disease Models, Animal
 Lung Diseases Antibodies  >68 publications with ARG and Lung Diseases
 Nervous System Diseases Antibodies  >65 publications with ARG and Nervous System Diseases
 Necrosis Antibodies  >50 publications with ARG and Necrosis
 Immune System Diseases Antibodies  >49 publications with ARG and Immune System Diseases
 Cardiovascular Diseases Antibodies  >47 publications with ARG and Cardiovascular Diseases
 Asthma Antibodies  >31 publications with ARG and Asthma
 Fibrosis Antibodies  >31 publications with ARG and Fibrosis
 Liver Diseases Antibodies  >27 publications with ARG and Liver Diseases

Organs/Tissues associated with anti-ARG antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >102 publications with ARG and Lung
 Brain Antibodies  >100 publications with ARG and Brain
 Liver Antibodies  >77 publications with ARG and Liver
 Small Intestine Antibodies  >41 publications with ARG and Small Intestine
 Spleen Antibodies  >33 publications with ARG and Spleen
 Eye Antibodies  >27 publications with ARG and Eye
 Colon Antibodies  >23 publications with ARG and Colon
 Connective Tissue Antibodies  >9 publications with ARG and Connective Tissue
 Pancreas Antibodies  >8 publications with ARG and Pancreas
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