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Lactate Dehydrogenase B (LDHB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025677
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2025677 is an antibody targeting LDHB. Also known as Monoclonal Antibody to Lactate Dehydrogenase B (LDHB). Reactive in Rat, Human, Mouse. Host: Mouse. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lactate Dehydrogenase B (LDHB), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Lactate Dehydrogenase B (LDHB)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-MATLKE KLIA PVADDETAVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCTIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
1#
Host
Mouse
Species Reactivity
Rat, Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
1mg/mL (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against LDHB. It has beenselected for its ability to recognize LDHB in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: LDHB Clone #1# (MBS2025677)
Immunogen: LDHB (MBS2010624)
Matching Pairs
Unconjugated Antibody: LDHB Clone #1# (MBS2025677)
APC-CY7 Conjugated Antibody: LDHB Clone #1# (MBS2058054)
Molecular Weight
36,612 Da
3D Structure
ModBase 3D Structure for P42123
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Human, Rat
Conjugate
No Conjugate
Immunogen
Recombinant LDHB (Met1-Leu334) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058058
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-LDHB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-LDHB antibody
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-LDHB antibody

Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Human Jurkat Cells.
Mouse Anti-LDHB Monoclonal Antibody – Cat# MBS2025677

Western Blot (WB) of anti-LDHB antibody

Western Blot: Sample: Recombinant LDHB, Rat.
Mouse Anti-LDHB Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025677

Other Notes

Small volumes of anti-LDHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase B chain isoform Ldhbx
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
Ldhb
NCBI Official Synonym Symbols
Ldh2
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H
UniProt Gene Name
Ldhb
UniProt Synonym Gene Names
Ldh-2; Ldh2; LDH-B; LDH-H
NCBI Protein Information
L-lactate dehydrogenase B chain

NCBI Summary for LDHB

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016]
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UniProt Comments for LDHB

LDH-B: an enzyme that participates in the final step of anaerobic glycolysis. Catalyzes the conversion of (S)-lactate + NAD+ into pyruvate + NADH.

Protein type: Amino Acid Metabolism - cysteine and methionine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 4q44

Cellular Component: cytosol; membrane; membrane raft; mitochondrion; myelin sheath

Molecular Function: identical protein binding; kinase binding; L-lactate dehydrogenase activity; lactate dehydrogenase activity; NAD binding; protein binding

Biological Process: carbohydrate metabolic process; lactate metabolic process; NAD metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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