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Peptidase Inhibitor 3 (PI3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025786
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2025786 is an antibody targeting PI3. Also known as Monoclonal Antibody to Peptidase Inhibitor 3, Skin Derived (PI3). Reactive in Human, Pig. Host: Mouse. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Peptidase Inhibitor 3 (PI3), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Peptidase Inhibitor 3, Skin Derived (PI3)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C8
Host
Mouse
Species Reactivity
Human, Pig
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Purification: Protein A + Protein G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Matching Pairs
Unconjugated Antibody: PI3 Clone #C8 (MBS2025786)
APC-CY7 Conjugated Antibody: PI3 Clone #C8 (MBS2068566)
Matching Pairs
Unconjugated Antibody: PI3 Clone #C8 (MBS2025786)
PE Conjugated Antibody: PI3 Clone #C8 (MBS2068568)
OMIM
Z18538 mRNA
3D Structure
ModBase 3D Structure for P19957
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Porcine
Immunogen
Recombinant PI3 (Phe41~Gln117) expressed in E.coli ( MBS2012194 ).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PI3 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-PI3 antibody
Western blotting: 0.01-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-PI3 antibody

Western Blot:
Sample: Recombinant PI3, Human.
Mouse Anti-PI3 Monoclonal Antibody – Cat# MBS2025786

Immunohistochemistry (IHC) of anti-PI3 antibody

DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human PI3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-PI3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025786

Immunohistochemistry (IHC) of anti-PI3 antibody

DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human PI3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-PI3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025786

Other Notes

Small volumes of anti-PI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PI3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
elafin preproprotein
NCBI Official Synonym Full Names
peptidase inhibitor 3
NCBI Official Symbol
PI3
NCBI Official Synonym Symbols
ESI; WAP3; SKALP; WFDC14; cementoin
UniProt Protein Name
Elafin
UniProt Synonym Protein Names
Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14
Protein Family
UniProt Gene Name
PI3
UniProt Synonym Gene Names
WAP3; WFDC14; ESI; PI-3; SKALP
NCBI Protein Information
elafin

NCBI Summary for PI3

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014]
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UniProt Comments for PI3

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cornified envelope; cytosol; extracellular region; proteinaceous extracellular matrix

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity; structural constituent of epidermis

Biological Process: antimicrobial humoral response; copulation; peptide cross-linking
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PI3 antibody

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