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Interleukin 18 (IL18) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025857
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MBS2025857 is an antibody targeting IL18. Also known as Monoclonal Antibody to Interleukin 18 (IL18). Host: Mouse. More details

$200 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 18 (IL18), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Interleukin 18 (IL18)
Product Gene Name
anti-IL18 antibody
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C14
Host
Mouse
Form/Format
Liquid; Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: IL18 Clone #C14 (MBS2025857)
Immunogen: IL18 (MBS2010987)
Matching Pairs
Unconjugated Antibody: IL18 Clone #C14 (MBS2025857)
APC-CY7 Conjugated Antibody: IL18 Clone #C14 (MBS2036559)
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OMIM
D49950 mRNA
3D Structure
ModBase 3D Structure for Q14116
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Monoclonal antibody preparation
Immunogen
Recombinant IL18 (Tyr37~Asp193) expressed in E.coli
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-IL18 antibody
Western Blot (WB); Immunohistochemistry (IHC)
Application Notes for anti-IL18 antibody
Western blotting: 0.01-2ug/mL;
Immunohistochemistry: 5-40ug/mL;
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-IL18 antibody

Western Blot; Sample: HepG2 cell lysate Primary Ab: 2ug/ml Mouse Anti-Human IL18 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-IL18 Monoclonal Antibody – Cat# MBS2025857

Western Blot (WB) of anti-IL18 antibody

Western Blot;
Samples:
Lane 1: HepG2 cell lysate;
Lane 2: Hela cell lysate;
Primary Ab: 0.2ug/ml Mouse Anti-Human IL18 Antibody
Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-IL18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025857

Western Blot (WB) of anti-IL18 antibody

Western Blot;
Sample: THP1 cell lysate
Primary Ab: 0.2ug/ml Mouse Anti-Human IL18 Antibody
Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-IL18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS2025857

Immunohistochemistry (IHC) of anti-IL18 antibody

DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 40ug/ml Mouse Anti-Human IL18 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-IL18 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025857

Immunohistochemistry (IHC) of anti-IL18 antibody

DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 40ug/ml Mouse Anti-Human IL18 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035)
Mouse Anti-IL18 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025857

Other Notes

Small volumes of anti-IL18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL18. It may not necessarily be applicable to this product.

NCBI GI #
342349321
NCBI GeneID
3606
NCBI Accession #
NP_001230140.1
UniProt Primary Accession #
Q14116
UniProt Secondary Accession #
O75599; Q6FGY3; Q6WWJ7
UniProt Related Accession #
Q14116
GenBank Nucleotide #
NM_001243211.1
NCBI Official Full Name
interleukin-18 isoform 2
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
IL18
NCBI Official Synonym Symbols
IGIF; IL-18; IL-1g; IL1F4
UniProt Protein Name
Interleukin-18
UniProt Synonym Protein Names
Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family
Interleukin
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
NCBI Protein Information
interleukin-18

NCBI Summary for IL18

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
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UniProt Comments for IL18

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: activation of protein kinase B activity; angiogenesis; cell-cell signaling; chemokine biosynthetic process; cholesterol homeostasis; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; inflammatory response; interferon-gamma biosynthetic process; interleukin-13 biosynthetic process; interleukin-2 biosynthetic process; lipopolysaccharide-mediated signaling pathway; MAPK cascade; natural killer cell activation; negative regulation of myoblast differentiation; positive regulation of activated T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of smooth muscle cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; regulation of cell adhesion; sleep; T-helper 1 type immune response; type 2 immune response
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Research Articles on IL18

1. an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL18 antibody

 Reference Product  PubMed Publications
 CASP1 antibody  >443 publications with IL18 and CASP1
 IL10 antibody  >274 publications with IL18 and IL10
 IL18R1 antibody  >215 publications with IL18 and IL18R1
 IL13 antibody  >83 publications with IL18 and IL13
 IL6 antibody  >58 publications with IL18 and IL6
 IL18RAP antibody  >29 publications with IL18 and IL18RAP
 IFNG antibody  >20 publications with IL18 and IFNG
 IL18BP antibody  >17 publications with IL18 and IL18BP
 IL1B antibody  >17 publications with IL18 and IL1B
 CCR5 antibody  >15 publications with IL18 and CCR5

Pathways associated with anti-IL18 antibody

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram

Diseases associated with anti-IL18 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1061 publications with IL18 and Inflammation
 Necrosis Antibodies  >731 publications with IL18 and Necrosis
 Neoplasms Antibodies  >500 publications with IL18 and Neoplasms
 Lung Diseases Antibodies  >301 publications with IL18 and Lung Diseases
 Kidney Diseases Antibodies  >251 publications with IL18 and Kidney Diseases
 Hypersensitivity Antibodies  >243 publications with IL18 and Hypersensitivity
 Arthritis, Rheumatoid Antibodies  >221 publications with IL18 and Arthritis, Rheumatoid
 Liver Diseases Antibodies  >205 publications with IL18 and Liver Diseases
 Heart Diseases Antibodies  >177 publications with IL18 and Heart Diseases
 Hypersensitivity, Immediate Antibodies  >174 publications with IL18 and Hypersensitivity, Immediate

Organs/Tissues associated with anti-IL18 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >363 publications with IL18 and Liver
 Lung Antibodies  >290 publications with IL18 and Lung
 Skin Antibodies  >197 publications with IL18 and Skin
 Brain Antibodies  >190 publications with IL18 and Brain
 Bone Antibodies  >190 publications with IL18 and Bone
 Heart Antibodies  >185 publications with IL18 and Heart
 Intestine Antibodies  >149 publications with IL18 and Intestine
 Bone Marrow Antibodies  >127 publications with IL18 and Bone Marrow
 Muscle Antibodies  >120 publications with IL18 and Muscle
 Connective Tissue Antibodies  >60 publications with IL18 and Connective Tissue
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