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Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025893
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2025893 is an antibody targeting H3. Also known as Polyclonal Antibody to Histone H3 (H3). Reactive in Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine… More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Histone H3 (H3)
Product Gene Name
anti-H3 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.5mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: H3 (MBS2025893)
Immunogen: H3 (MBS2031345)
Matching Pairs
Unconjugated Antibody: H3 (MBS2025893)
APC-CY7 Conjugated Antibody: H3 (MBS2040536)
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Molecular Weight
15,406 Da
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Mouse; Rat; Cavia; Simian; Canine; Porcine; Bovine; Ovine; Equine; Gallus
Immunogen
Recombinant Histone H3 (Met1~Ala136) expressed in E.coli (MBS2031345)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-H3 antibody
Western blotting: 0.01-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunofluorescence: 5-20ug/mL;
Optimal working dilutions must be determined by end user.

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple: Rat Skeletal Muscle Tissue.
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P;Simple:Rat Spinal Cord Tissue
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple: Rat Kidney Tissue
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P;Simple: Rat Adrenal Tissue
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P;Simple:Rat Placenta Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple: Rat Stomach Tissue.)
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple: Rat Small Intestine Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple: Rat Heart Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple:Rat Pancreas Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P; Simple: Rat Brain Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

FITC staining on IHC-P Simple:Rat Testis Tissue
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

Vector Red staining on IHC-P; Samples: Human Skin Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human H3 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

Vector Red staining on IHC-P; Samples: Human Small Intestine Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human H3 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Western Blot (WB) of anti-H3 antibody

Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Rat Liver lysate; Lane4: Rat Cerebrum lysate Primary Ab: 1ug/ml Rabbit Anti-Multi- species H3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-H3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2025893

Immunohistochemistry (IHC) of anti-H3 antibody

Figure: FITC staining on IHC-P; Sample: Rat Liver Tissue.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2025893

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
3273350
NCBI Accession #
BAA31218.1
NCBI Official Full Name
histone H3
UniProt Protein Name
Histone H3.3
Protein Family
Tyrosine phosphatase
UniProt Gene Name
H3
UniProt Entry Name
H33_TOBAC

UniProt Comments for H3

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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