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Elastase 3a (ELA3A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2026469
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MBS2026469 is an antibody targeting ELA3A. Also known as Polyclonal Antibody to Elastase 3A (ELA3A). Reactive in Human, Mouse, Rat, Pig, Bovine. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Elastase 3A (ELA3A), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Elastase 3A (ELA3A)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Pig, Bovine
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against ELA3A. It has beenselected for its ability to recognize ELA3A in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: ELA3A (MBS2026469)
Immunogen: ELA3A (MBS2028875)
Matching Pairs
Unconjugated Antibody: ELA3A (MBS2026469)
APC-CY7 Conjugated Antibody: ELA3A (MBS2074141)
Molecular Weight
29,489 Da
3D Structure
ModBase 3D Structure for P09093
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
ELA3A (Ser16~His270)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse, Bovine
Conjugate
No Conjugate
Immunogen
Recombinant ELA3A (Ser16~His270) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074143
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-ELA3A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-ELA3A antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ELA3A antibody

Western Blot: Sample: Mouse Pancreas lysate; Primary Ab: 2ug/ml Rabbit Anti-Human ELA3A Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
Rabbit Anti-ELA3A Polyclonal Antibody – Cat# MBS2026469

Western Blot (WB) of anti-ELA3A antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-ELA3A Polyclonal Antibody, Western Blot (WB) – Cat# MBS2026469

Immunohistochemistry (IHC) of anti-ELA3A antibody

DAB staining on IHC-P. Samples: Human Tissue)
Rabbit Anti-ELA3A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026469

Other Notes

Small volumes of anti-ELA3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ELA3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chymotrypsin-like elastase family member 3A preproprotein
NCBI Official Synonym Full Names
chymotrypsin like elastase family member 3A
NCBI Official Symbol
CELA3A
NCBI Official Synonym Symbols
ELA3; ELA3A
UniProt Protein Name
Chymotrypsin-like elastase family member 3A
UniProt Synonym Protein Names
Elastase IIIA; Elastase-3A; Protease E
UniProt Gene Name
CELA3A
UniProt Synonym Gene Names
ELA3; ELA3A
NCBI Protein Information
chymotrypsin-like elastase family member 3A

NCBI Summary for ELA3A

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]
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UniProt Comments for ELA3A

CELA3A: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.70; Protease

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase activity

Biological Process: cholesterol metabolic process; digestion; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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