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Nucleobindin 2 (NUCB2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2026571
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2026571 is an antibody targeting NUCB2. Also known as Polyclonal Antibody to Nucleobindin 2 (NUCB2). Reactive in Human, Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleobindin 2 (NUCB2), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Nucleobindin 2 (NUCB2)
Product Gene Name
anti-NUCB2 antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- VPIDVD KTKVHNTEPV ENARIEPPDT GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPNTF ESRDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY ESIIAIQENE LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK LQQGIAPSGP AGELKFEPHT
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against NUCB2. It has beenselected for its ability to recognize NUCB2 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: NUCB2 (MBS2026571)
Immunogen: NUCB2 (MBS2029335)
Matching Pairs
Unconjugated Antibody: NUCB2 (MBS2026571)
APC-CY7 Conjugated Antibody: NUCB2 (MBS2070445)
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Molecular Weight
50,304 Da
3D Structure
ModBase 3D Structure for P81117
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
NUCB2 (Val25~Thr420)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant NUCB2 (Val25~Thr420) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2070447
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NUCB2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-NUCB2 antibody
Western blotting: 0.5-5ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-NUCB2 antibody

Western Blot: Sample: Mouse Stomach Tissue.
Rabbit Anti-NUCB2 Polyclonal Antibody – Cat# MBS2026571

Western Blot (WB) of anti-NUCB2 antibody

Western Blot: Sample: Recombinant NUCB2, Mouse.
Rabbit Anti-NUCB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2026571

Immunohistochemistry (IHC) of anti-NUCB2 antibody

DAB staining on IHC-P; Samples: Mouse Ovary Tissue.
Rabbit Anti-NUCB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026571

Immunohistochemistry (IHC) of anti-NUCB2 antibody

DAB staining on IHC-P; Samples: Mouse Intestine Tissue
Rabbit Anti-NUCB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026571

Immunohistochemistry (IHC) of anti-NUCB2 antibody

DAB staining on IHC-P; Samples: Mouse Uterus Tissue
Rabbit Anti-NUCB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026571

Other Notes

Small volumes of anti-NUCB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NUCB2. It may not necessarily be applicable to this product.

NCBI GI #
194440700
NCBI GeneID
53322
NCBI Accession #
NP_001123951.1
UniProt Primary Accession #
P81117
UniProt Related Accession #
P81117
GenBank Nucleotide #
NM_001130479.2
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
Nucb2
NCBI Official Synonym Symbols
Nefa; Calnuc; AI607786; Nesfatin-1
UniProt Protein Name
Nucleobindin-2
UniProt Synonym Protein Names
DNA-binding protein NEFA; Prepronesfatin
Protein Family
Nucleobindin
UniProt Gene Name
Nucb2
UniProt Synonym Gene Names
Nefa
NCBI Protein Information
nucleobindin-2

UniProt Comments for NUCB2

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 7|7 F1

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum-Golgi intermediate compartment; extracellular region; extracellular space; Golgi apparatus; Golgi medial cisterna; membrane; nuclear outer membrane; nucleus

Molecular Function: calcium ion binding; DNA binding; metal ion binding; tumor necrosis factor receptor binding

Biological Process: cellular calcium ion homeostasis; negative regulation of appetite; negative regulation of cAMP biosynthetic process; negative regulation of fat cell differentiation; negative regulation of insulin receptor signaling pathway; positive regulation of fatty acid oxidation
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Research Articles on NUCB2

1. Tension receptor mechanosensitivity was unaffected by nesfatin-1 in SLD and fasted mice, but was inhibited in HFD mice. In conclusion, Nesfatin-1 modulates gastric vagal afferent mechanosensitivity in a nutritional state dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-NUCB2 antibody

 Disease Name  Pubmed Publications
 Weight Gain Antibodies  >11 publications with NUCB2 and Weight Gain
 Insulin Resistance Antibodies  >7 publications with NUCB2 and Insulin Resistance
 Disease Models, Animal Antibodies  >4 publications with NUCB2 and Disease Models, Animal
 Necrosis Antibodies  >4 publications with NUCB2 and Necrosis
 Breast Neoplasms Antibodies  >4 publications with NUCB2 and Breast Neoplasms
 Inflammation Antibodies  >4 publications with NUCB2 and Inflammation
 Liver Diseases Antibodies  >4 publications with NUCB2 and Liver Diseases
 Carcinoma Antibodies  >3 publications with NUCB2 and Carcinoma
 Prostatic Neoplasms Antibodies  >3 publications with NUCB2 and Prostatic Neoplasms
 Fatty Liver Antibodies  >2 publications with NUCB2 and Fatty Liver

Organs/Tissues associated with anti-NUCB2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >61 publications with NUCB2 and Brain
 Blood Antibodies  >29 publications with NUCB2 and Blood
 Pancreas Antibodies  >17 publications with NUCB2 and Pancreas
 Liver Antibodies  >13 publications with NUCB2 and Liver
 Ovary Antibodies  >6 publications with NUCB2 and Ovary
 Pituitary Gland Antibodies  >6 publications with NUCB2 and Pituitary Gland
 Muscle Antibodies  >5 publications with NUCB2 and Muscle
 Connective Tissue Antibodies  >5 publications with NUCB2 and Connective Tissue
 Prostate Antibodies  >3 publications with NUCB2 and Prostate
 Testis Antibodies  >2 publications with NUCB2 and Testis
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