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Nestin (NES) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2026576
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MBS2026576 is an antibody targeting NES. Also known as Polyclonal Antibody to Nestin (NES). Reactive in Human. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nestin, Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Nestin (NES)
Product Gene Name
anti-NES antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAR RLGEAWRGAV RGYQERVAHM ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV LSPTSLPSPL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against NES. It has beenselected for its ability to recognize NES in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: NES (MBS2026576)
Immunogen: NES (MBS2029165)
Matching Pairs
Unconjugated Antibody: NES (MBS2026576)
APC-CY7 Conjugated Antibody: NES (MBS2043103)
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Molecular Weight
177,439 Da
OMIM
AL590666 Genomic DNA
3D Structure
ModBase 3D Structure for P48681
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
NES (Leu178~Pro399)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant NES (Leu178~Pro399) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043107
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NES antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-NES antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-NES antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-NES Polyclonal Antibody – Cat# MBS2026576

Immunohistochemistry (IHC) of anti-NES antibody

DAB staining on IHC-P; Samples: Human Glioma Tissue)
Rabbit Anti-NES Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026576

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
NCBI Protein Information
nestin

NCBI Summary for NES

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

UniProt Comments for NES

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; extracellular matrix; intermediate filament; intermediate filament cytoskeleton

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: brain development; central nervous system development; embryonic camera-type eye development; G2/M transition of mitotic cell cycle; negative regulation of catalytic activity; negative regulation of protein binding; positive regulation of intermediate filament depolymerization; positive regulation of neural precursor cell proliferation; stem cell proliferation
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Research Articles on NES

1. Study clarified the relationship between Nestin overexpression and poor prognosis in recurrent human hepatocellular carcinoma (HCC) patients receiving FOLFOX regimen, and its association with chemoresistance and epithelial-mesenchymal transition in HCC cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 MYCN antibody  >6 publications with NES and MYCN
 EGF antibody  >4 publications with NES and EGF
 VSX2 antibody  >1 publications with NES and VSX2
 CDK5 antibody  >1 publications with NES and CDK5

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >365 publications with NES and Nervous System Diseases
 Neoplasms Antibodies  >223 publications with NES and Neoplasms
 Brain Diseases Antibodies  >216 publications with NES and Brain Diseases
 Seizures Antibodies  >122 publications with NES and Seizures
 Cardiovascular Diseases Antibodies  >69 publications with NES and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >52 publications with NES and Breast Neoplasms
 Disease Models, Animal Antibodies  >38 publications with NES and Disease Models, Animal
 Pain Antibodies  >37 publications with NES and Pain
 Heart Diseases Antibodies  >30 publications with NES and Heart Diseases
 Drug Toxicity Antibodies  >29 publications with NES and Drug Toxicity

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >191 publications with NES and Brain
 Nerve Antibodies  >109 publications with NES and Nerve
 Muscle Antibodies  >72 publications with NES and Muscle
 Heart Antibodies  >57 publications with NES and Heart
 Eye Antibodies  >45 publications with NES and Eye
 Vascular Antibodies  >44 publications with NES and Vascular
 Embryonic Tissue Antibodies  >40 publications with NES and Embryonic Tissue
 Kidney Antibodies  >37 publications with NES and Kidney
 Lung Antibodies  >29 publications with NES and Lung
 Skin Antibodies  >26 publications with NES and Skin
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