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O-6-Methylguanine DNA Methyltransferase (MGMT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2026596
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MBS2026596 is an antibody targeting MGMT. Also known as Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT). Reactive in Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
O-6-Methylguanine DNA Methyltransferase (MGMT), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
Product Gene Name
anti-MGMT antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.31mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: MGMT (MBS2026596)
Immunogen: MGMT (MBS2029209)
Matching Pairs
Unconjugated Antibody: MGMT (MBS2026596)
APC-CY7 Conjugated Antibody: MGMT (MBS2062223)
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3D Structure
ModBase 3D Structure for P24528
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant MGMT (Met1~Gly209) expressed in E.coli ( MBS2029209 ).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-MGMT antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-MGMT antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-MGMT antibody

Figure. Western Blot; Sample: Recombinant MGMT, Rat.
Rabbit Anti-MGMT Polyclonal Antibody – Cat# MBS2026596

Other Notes

Small volumes of anti-MGMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MGMT. It may not necessarily be applicable to this product.

NCBI GI #
6981202
NCBI GeneID
25332
NCBI Accession #
NP_036993.1
UniProt Primary Accession #
P24528
UniProt Related Accession #
P24528
GenBank Nucleotide #
NM_012861.1
NCBI Official Full Name
methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
Mgmt
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family
Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
Mgmt
UniProt Synonym Gene Names
MGMT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase

NCBI Summary for MGMT

enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]

UniProt Comments for MGMT

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Apoptosis; DNA repair, damage; EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: membrane; nucleoplasm; nucleus

Molecular Function: calcium ion binding; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity

Biological Process: cellular response to DNA damage stimulus; DNA dealkylation involved in DNA repair; DNA repair; methylation; negative regulation of apoptosis; positive regulation of DNA repair; regulation of caspase activity; response to drug; response to ethanol; response to folic acid; response to organic cyclic compound; response to toxin
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Research Articles on MGMT

1. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MGMT antibody

 Reference Product  PubMed Publications
 Tp53 antibody  >128 publications with MGMT and Tp53
 Kras antibody  >88 publications with MGMT and Kras
 Nras antibody  >4 publications with MGMT and Nras
 Myb antibody  >1 publications with MGMT and Myb
 Zeb1 antibody  >1 publications with MGMT and Zeb1
 Mmp7 antibody  >1 publications with MGMT and Mmp7

Diseases associated with anti-MGMT antibody

 Disease Name  Pubmed Publications
 Glioma Antibodies  >1103 publications with MGMT and Glioma
 Nervous System Diseases Antibodies  >951 publications with MGMT and Nervous System Diseases
 Brain Neoplasms Antibodies  >925 publications with MGMT and Brain Neoplasms
 Glioblastoma Antibodies  >858 publications with MGMT and Glioblastoma
 Carcinoma Antibodies  >482 publications with MGMT and Carcinoma
 Recurrence Antibodies  >243 publications with MGMT and Recurrence
 Adenocarcinoma Antibodies  >181 publications with MGMT and Adenocarcinoma
 Lung Neoplasms Antibodies  >151 publications with MGMT and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >149 publications with MGMT and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >127 publications with MGMT and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-MGMT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1030 publications with MGMT and Brain
 Prostate Antibodies  >30 publications with MGMT and Prostate
 Ovary Antibodies  >21 publications with MGMT and Ovary
 Heart Antibodies  >6 publications with MGMT and Heart
 Connective Tissue Antibodies  >1 publications with MGMT and Connective Tissue
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