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R-Spondin 3 (RSPO3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2026604
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2026604 is an antibody targeting RSPO3. Also known as Polyclonal Antibody to R-Spondin 3 (RSPO3). Reactive in Human, Rat, Pig. Host: Rabbit. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
R-Spondin 3 (RSPO3), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to R-Spondin 3 (RSPO3)
Product Gene Name
anti-RSPO3 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Pig
Form/Format
Liquid
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against RSPO3. It has beenselected for its ability to recognize RSPO3 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: RSPO3 (MBS2026604)
Immunogen: RSPO3 (MBS2029971)
Matching Pairs
Unconjugated Antibody: RSPO3 (MBS2026604)
APC-CY7 Conjugated Antibody: RSPO3 (MBS2083717)
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Molecular Weight
33,233 Da
OMIM
AF251057 mRNA
3D Structure
ModBase 3D Structure for Q9BXY4
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
RSPO3 (Gln22~His272)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Pig
Conjugate
No Conjugate
Immunogen
Recombinant RSPO3 (Gln22~His272) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083719
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-RSPO3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-RSPO3 antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-RSPO3 antibody

Western Blot: Sample: Porcine Small intestine lysate; Primary Ab: 3ug/ml Rabbit Anti-Human RSPO3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-RSPO3 Polyclonal Antibody – Cat# MBS2026604

Western Blot (WB) of anti-RSPO3 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-RSPO3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2026604

Immunohistochemistry (IHC) of anti-RSPO3 antibody

DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue)
Rabbit Anti-RSPO3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2026604

Other Notes

Small volumes of anti-RSPO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RSPO3. It may not necessarily be applicable to this product.

NCBI GI #
18490982
NCBI GeneID
84870
NCBI Accession #
NP_116173.2
UniProt Primary Accession #
Q9BXY4
UniProt Secondary Accession #
Q5VTV4; Q96K87; B2RC27
UniProt Related Accession #
Q9BXY4
GenBank Nucleotide #
NM_032784.4
NCBI Official Full Name
R-spondin-3
NCBI Official Synonym Full Names
R-spondin 3
NCBI Official Symbol
RSPO3
NCBI Official Synonym Symbols
PWTSR; THSD2; CRISTIN1
UniProt Protein Name
R-spondin-3
UniProt Synonym Protein Names
Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2
Protein Family
R-spondin
UniProt Gene Name
RSPO3
UniProt Synonym Gene Names
PWTSR; THSD2; hPWTSR; hRspo3
NCBI Protein Information
R-spondin-3

NCBI Summary for RSPO3

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]
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UniProt Comments for RSPO3

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular region

Molecular Function: frizzled binding; heparin binding; receptor binding

Biological Process: blood vessel remodeling; positive regulation of Wnt receptor signaling pathway; regulation of Wnt receptor signaling pathway; sprouting angiogenesis; Wnt signaling pathway
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Research Articles on RSPO3

1. Colorectal cancer cell lines identified VACO6 cells as a carrier of a canonical PTPRK(e1)-RSPO3(e2) fusion; cell line displayed marked in vitro and in vivo sensitivity to WNT blockade by the porcupine inhibitor LGK974. Long-term treatment of VACO6 cells with LGK974 led to the emergence of a resistant population carrying two frameshift deletions of the WNT pathway inhibitor AXIN1, with consequent protein loss.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RSPO3 antibody

 Reference Product  PubMed Publications
 FURIN antibody  >3 publications with RSPO3 and FURIN
 SDC4 antibody  >2 publications with RSPO3 and SDC4
 LRP6 antibody  >1 publications with RSPO3 and LRP6
 WNT11 antibody  >1 publications with RSPO3 and WNT11
 MYF5 antibody  >1 publications with RSPO3 and MYF5

Pathways associated with anti-RSPO3 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
 Regulation Of FZD By Ubiquitination Pathway antibodies  Regulation Of FZD By Ubiquitination Pathway Diagram

Diseases associated with anti-RSPO3 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with RSPO3 and Disease Models, Animal
 Bone Diseases, Metabolic Antibodies  >2 publications with RSPO3 and Bone Diseases, Metabolic
 Breast Neoplasms Antibodies  >1 publications with RSPO3 and Breast Neoplasms

Organs/Tissues associated with anti-RSPO3 antibody

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >5 publications with RSPO3 and Connective Tissue
 Brain Antibodies  >5 publications with RSPO3 and Brain
 Embryonic Tissue Antibodies  >4 publications with RSPO3 and Embryonic Tissue
 Adipose Tissue Antibodies  >3 publications with RSPO3 and Adipose Tissue
 Lung Antibodies  >3 publications with RSPO3 and Lung
 Heart Antibodies  >3 publications with RSPO3 and Heart
 Placenta Antibodies  >2 publications with RSPO3 and Placenta
 Prostate Antibodies  >2 publications with RSPO3 and Prostate
 Intestine Antibodies  >1 publications with RSPO3 and Intestine
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