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GroEL Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203023

GroEL protein is the major heat shock protein of E Coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition.… More details

$210 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GroEL, Recombinant Protein
Full Product Name
GroEL, 1-548aa, E Coli, E Coli
Product Gene Name
Product Synonym Names
groL; groEL; mopA; Protein Cpn60; 60 kDa chaperonin; groEL protein; chaperonin groel
Sequence
MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM
Host
E Coli
Form/Format
Liquid. In 25 mM Tris-HCl (pH 7.5) 100mM NaCl, 5mM DTT, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at 20°C to -80°C. Avoid repeated freezing and thawing cycles.
Molecular Weight
57.3kDa (548aa), confirmed by MALDI-TOF.
3D Structure
ModBase 3D Structure for P0A6F5
Antigen Species
E Coli

Product Categories/Family for GroEL recombinant protein

Applications Tested/Suitable for GroEL recombinant protein
SDS-PAGE

SDS-Page of GroEL recombinant protein

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
GroEL Recombinant Protein – Cat# MBS203023

Product References and Citations for GroEL recombinant protein

Hemmingsen,S.M., et al (1988) Nature. 333 (6171) 330-334; Braig,K., et al (1994) Nature 371 (6498) 578-586; Chen,L and Singler,P.B. (1999) Cell 99 (7) 757-768;

Other Notes

Small volumes of GroEL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GroEL purchase

MBS203023 is a recombinant protein targeting GroEL. Also known as GroEL, 1-548aa, E Coli, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GroEL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
Cpn60 chaperonin GroEL, large subunit of GroESL
NCBI Official Symbol
groL
NCBI Official Synonym Symbols
ECK4137; groEL; JW4103; mopA
UniProt Protein Name
60 kDa chaperonin
UniProt Synonym Protein Names
GroEL protein
Protein Family
UniProt Gene Name
groL
NCBI Protein Information
Cpn60 chaperonin GroEL, large subunit of GroESL

NCBI Summary for GroEL

groL is an essential gene. [More information is available at EcoGene: EG10599]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10599].

UniProt Comments for GroEL

Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GroEL recombinant protein

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