• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DNAK Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203030

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of… More details

$210 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dnak, Recombinant Protein
Full Product Name
Dnak, 508-638aa, E Coli, E Coli
Product Gene Name
Product Synonym Names
Dnak (508-638); A lid covering the substrate; Heat shock protein 70; Heat shock 70 kDa protein; HSP70; Chaperone protein dnaK; Chaperone Hsp 70; Co chaperone with DnaJ; dnaK; Heat shock 70 kDa protein
Sequence
MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
Host
E Coli
Form/Format
Liquid. In 25 mM Tris-HCl Buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
14.6kDa (132aa)
3D Structure
ModBase 3D Structure for P0A6Y8
Antigen Species
E Coli

Product Categories/Family for Dnak recombinant protein

Applications Tested/Suitable for Dnak recombinant protein
SDS-PAGE

Product References and Citations for Dnak recombinant protein

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; Zhu et al., (1996) Science 272, 1606-1614.; Naoki tanaka., et al (2002) PNAS 26 (99)15398-15403;

Other Notes

Small volumes of Dnak recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dnak purchase

MBS203030 is a recombinant protein targeting Dnak. Also known as Dnak, 508-638aa, E Coli, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Dnak. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
chaperone Hsp70, with co-chaperone DnaJ
NCBI Official Symbol
dnaK
NCBI Official Synonym Symbols
ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
UniProt Protein Name
Chaperone protein DnaK
UniProt Synonym Protein Names
HSP70; Heat shock 70 kDa protein; Heat shock protein 70
Protein Family
UniProt Gene Name
dnaK
UniProt Synonym Gene Names
groP; grpF; seg
NCBI Protein Information
chaperone Hsp70, with co-chaperone DnaJ

NCBI Summary for Dnak

ATP-regulated binding and release of polypeptide substrates. [More information is available at EcoGene: EG10241]. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity . [More information is available at EcoCyc: EG10241].

UniProt Comments for Dnak

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000