3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

DnaJ Recombinant Protein

Name: DnaJ Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203031
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203031

DnaJ, Heat shock protein, functions in association with DnaK (Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DnaJ, Recombinant Protein

Full Product Name

DnaJ, 1-376aa, E Coli, E Coli

Product Gene Name

DnaJ recombinant protein

Product Synonym Names

DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ (1-376aa), Heat shock 40 kDa protein 4, DnaJ protein homolog 2, HDJ-2, HSJ-2, HSDJ, DnaJ homolog subfamily A member 1

Sequence

MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR

Form/Format

Liquid. In 25 mM Tris-HCl Buffer (pH 8.8) containing 5 mM DTT, 100 mM NaCl, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freexing and thawing cycles.

Molecular Weight

41.1kDa (376aa), confirmed by MALDI-TOF.

Sequence Positions

1-376aa

3D Structure

ModBase 3D Structure for P08622

Tag

Non-Tagged

Product Categories/Family for DnaJ recombinant protein

Heat Shock Proteins

Applications Tested/Suitable for DnaJ recombinant protein

SDS-PAGE

Product References and Citations for DnaJ recombinant protein

Bardwell,J.C., et al (1986) J.Biol.Chem.261 (4);1782-1785; Ohki M.,et al (1986) J.Biol.Chem.261 (4);1778-1781; Zylicz M., et.al. (1989) EMBO J 8 (5)1601-8 ; Landry SJ., et al. (2003) Biochemistry 42 (17)4926-36;

Other Notes

Small volumes of DnaJ recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DnaJ purchase

MBS203031 is a recombinant protein targeting DnaJ. Also known as DnaJ, 1-376aa, E Coli, E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DnaJ. It may not necessarily be applicable to this product.

NCBI GI #

16128009

NCBI GeneID

944753

NCBI Accession #

NP_414556

UniProt Primary Accession #

P08622

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

chaperone Hsp40, DnaK co-chaperone

NCBI Official Symbol

dnaJ

NCBI Official Synonym Symbols

ECK0015; faa; groP; grpC; JW0014

UniProt Protein Name

Chaperone protein DnaJ

UniProt Synonym Protein Names

HSP40; Heat shock protein J

Protein Family

Chaperone protein

UniProt Gene Name

dnaJ

UniProt Synonym Gene Names

groP

NCBI Protein Information

chaperone Hsp40, DnaK co-chaperone

NCBI Summary for DnaJ

DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].

UniProt Comments for DnaJ

Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

Research Articles on DnaJ

1. These results suggest that, in the absence of interaction with Escherichia coli DnaJ, the N-terminal domain of the MS2 bacteriophage L lysis protein interferes with its ability to bind to its unknown target.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DnaJ recombinant protein

Reference Product	PubMed Publications
dnaK recombinant protein	>715 publications with DnaJ and dnaK
grpE recombinant protein	>403 publications with DnaJ and grpE
clpB recombinant protein	>75 publications with DnaJ and clpB
htpG recombinant protein	>10 publications with DnaJ and htpG
hscA recombinant protein	>8 publications with DnaJ and hscA
hscC recombinant protein	>5 publications with DnaJ and hscC
groL recombinant protein	>2 publications with DnaJ and groL
yegD recombinant protein	>1 publications with DnaJ and yegD