ubc7 Recombinant Protein – Cat# MBS203071

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Ubc7 (Ubiquitin Conjugating Enzyme 7) (ubc7) Recombinant Protein

Name: ubc7 Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS203071
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203071

Human ubquitin-conjugating enzyme 7 (ubcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ubc7 (ubiquitin Conjugating enzyme 7), Recombinant Protein

Full Product Name

ubc7 (ubiquitin Conjugating enzyme 7), 1-154 aa, Human, E Coli

Product Gene Name

ubc7 recombinant protein

Product Synonym Names

ubiquitin-conjugating enzyme E2L 3; uBE2G1; uBE2G; ubc7 (ubiquitin Conjugating enzyme 7); EC 6.3.2.19; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; E217K; uBC7; ubiquitin-conjugating enzyme E2 G1; ubiquitin-conjugating enzyme E2L 3 uBC 7; uBC7 homolog yeast; uBE 2G; ubiquitin conjugating enzyme E2 G1; ubiquitin conjugating enzyme E2G 1 (homologous to C; elegans uBC7); ubiquitin conjugating enzyme E2G 1 (uBC7 homolog C; elegans) antibody; ubiquitin conjugating enzyme E2G 1 (uBC7 homolog yeast); ubiquitin conjugating enzyme E2G 1; ubiquitin protein ligase G1

Sequence

MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

Host

E Coli

Form/Format

Liquid. In 50 mM HEPES (pH 7.5) 150 mM NaCl, 1mM DTT, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

17.9 kDa (154 aa), confirmed by MALDI-TOF.

OMIM

S81003 mRNA

3D Structure

ModBase 3D Structure for P68036

Domain

1-154aa

Product Categories/Family for ubc7 recombinant protein

Ubiquitination

Applications Tested/Suitable for ubc7 recombinant protein

SDS-PAGE

Product References and Citations for ubc7 recombinant protein

Ardley HC., et al. (2000) Biochim Biophys Acta. 1491 (1-3):57-64.; Moynihan TP., et al. (1998) Genomics. 51 (1):124-127.; Nuber u., et al (1996) J Biol Chem. 271 (5):2795-2800;

Other Notes

Small volumes of ubc7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ubc7 purchase

MBS203071 is a recombinant protein targeting ubc7. Also known as ubc7 (ubiquitin Conjugating enzyme 7), 1-154 aa, Human, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ubc7. It may not necessarily be applicable to this product.

NCBI GI #

4507789

NCBI GeneID

7332

NCBI Accession #

NP_003338

UniProt Primary Accession #

P68036

UniProt Secondary Accession #

P51966; P70653; Q9HAV1; B2R4A7; B4DDG1; B4DSZ4; E7EWS7

UniProt Related Accession #

P68036

GenBank Nucleotide #

NM_003347.3

NCBI Official Full Name

ubiquitin-conjugating enzyme E2 L3 isoform 1

NCBI Official Synonym Full Names

ubiquitin conjugating enzyme E2 L3

NCBI Official Symbol

UBE2L3

NCBI Official Synonym Symbols

E2-F1; L-UBC; UBCH7; UbcM4

UniProt Protein Name

Ubiquitin-conjugating enzyme E2 L3

UniProt Synonym Protein Names

E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3

Protein Family

Ubiquitin-conjugating enzyme

UniProt Gene Name

UBE2L3

UniProt Synonym Gene Names

UBCE7; UBCH7

NCBI Protein Information

ubiquitin-conjugating enzyme E2 L3

NCBI Summary for ubc7

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

UniProt Comments for ubc7

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

Research Articles on ubc7

1. Data suggest ordered binding of UBCH7-ubiquitin to E6AP Site 1 (for E6AP-Cys820/ubiquitin thioester formation) and E6AP Site 2 (for subsequent chain elongation); proximal indexation accounts for radially symmetric structure of E6AP, requirement for oligomerization in polyubiquitin chain formation, and mechanistic rationale for Cys820-ubiquitin thioester as platform in chain assembly. (E6AP = ubiquitin-protein ligase E6AP)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ubc7 recombinant protein

Reference Product	PubMed Publications
UBE3A recombinant protein	>6 publications with ubc7 and UBE3A
UBE2D2 recombinant protein	>5 publications with ubc7 and UBE2D2
ARIH2 recombinant protein	>2 publications with ubc7 and ARIH2
RNF19A recombinant protein	>1 publications with ubc7 and RNF19A

Pathways associated with ubc7 recombinant protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
BARD1 Signaling Events Pathway antibodies	BARD1 Signaling Events Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
E3 Ubiquitin Ligases Ubiquitinate Target Proteins Pathway antibodies	E3 Ubiquitin Ligases Ubiquitinate Target Proteins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Parkin-Ubiquitin Proteasomal System Pathway antibodies	Parkin-Ubiquitin Proteasomal System Pathway Diagram
Parkinson's Disease Pathway antibodies	Parkinson's Disease Pathway Diagram

Diseases associated with ubc7 recombinant protein

Disease Name	Pubmed Publications
Autoimmune Diseases Antibodies	>19 publications with ubc7 and Autoimmune Diseases
Immune System Diseases Antibodies	>17 publications with ubc7 and Immune System Diseases
Death Antibodies	>12 publications with ubc7 and Death
Inflammation Antibodies	>6 publications with ubc7 and Inflammation
Liver Diseases Antibodies	>6 publications with ubc7 and Liver Diseases
Necrosis Antibodies	>5 publications with ubc7 and Necrosis
Lung Diseases Antibodies	>3 publications with ubc7 and Lung Diseases
Hepatitis B, Chronic Antibodies	>3 publications with ubc7 and Hepatitis B, Chronic
Fibrosis Antibodies	>2 publications with ubc7 and Fibrosis
Congenital Abnormalities Antibodies	>2 publications with ubc7 and Congenital Abnormalities

Organs/Tissues associated with ubc7 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with ubc7 and Blood
Brain Antibodies	>11 publications with ubc7 and Brain
Liver Antibodies	>8 publications with ubc7 and Liver
Lung Antibodies	>5 publications with ubc7 and Lung
Muscle Antibodies	>5 publications with ubc7 and Muscle
Skin Antibodies	>4 publications with ubc7 and Skin
Nerve Antibodies	>4 publications with ubc7 and Nerve
Prostate Antibodies	>2 publications with ubc7 and Prostate
Heart Antibodies	>2 publications with ubc7 and Heart
Embryonic Tissue Antibodies	>2 publications with ubc7 and Embryonic Tissue