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SET7/9 Histone Methyltransferase (SETD7) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203073

SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SET7/9 Histone methyltransferase (SETD7), Recombinant Protein
Full Product Name
SET7/9 Histone methyltransferase, 1-366 aa, Human, E Coli
Product Gene Name
Product Synonym Names
SET domain-containing protein 7; SETD7; KIAA1717; KMT7; SET7; SET9; SET7/9 Histone methyltransferase; EC 2.1.1.43; Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; SET7/9; Lysine N-methyltransferase 7; Histone-lysine N-methyltransferase SETD7; SET domain-containing protein 7 FLJ21193; H3 K4 HMTase; H4 lysine-4 specific; Histone H3 K4 methyltransferase; Histone H3 lysine 4 specific methyltransferase; Histone H4-K4 methyltransferase; Histone lysine N methyltransferase H3 lysine 4 specific SET7; Histone-lysine N-methyltransferase; Lysine methyltransferase; SET 7; SET 7/9; SET 9; SET D7; SET domain containing protein 7; SET domain-containing protein 8
Sequence
MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK
Host
E Coli
Form/Format
Liquid. In 50 mM Tris-HCl Buffer (pH 7.5) 0.2M NaCl, 5mM DTT, 20%glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
40.7 kDa (366 aa), confirmed by MALDI-TOF.
OMIM
AF448510 mRNA
3D Structure
ModBase 3D Structure for Q8WTS6
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for SETD7 recombinant protein

Applications Tested/Suitable for SETD7 recombinant protein
SDS-PAGE

Product References and Citations for SETD7 recombinant protein

Xiao B., et al. (2003) Nature. 421 (6923):652-6.; Kwon T., et al. (2003) EMBO J. 22, 292-303.;

Other Notes

Small volumes of SETD7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETD7 purchase

MBS203073 is a recombinant protein targeting SETD7. Also known as SET7/9 Histone methyltransferase, 1-366 aa, Human, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SETD7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase SETD7 isoform 1
NCBI Official Synonym Full Names
SET domain containing lysine methyltransferase 7
NCBI Official Symbol
SETD7
NCBI Official Synonym Symbols
KMT7; SET7; SET9; SET7/9
UniProt Protein Name
Histone-lysine N-methyltransferase SETD7
UniProt Synonym Protein Names
Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; Lysine N-methyltransferase 7; SET domain-containing protein 7; SET7/9
UniProt Gene Name
SETD7
UniProt Synonym Gene Names
KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
NCBI Protein Information
histone-lysine N-methyltransferase SETD7

UniProt Comments for SETD7

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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