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Prolactin (PRL) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203093

Prolactin is a hormone synthesised and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation).Increased serum… More details

$230 / 0.02 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Prolactin (PRL), Active Protein
Full Product Name
Prolactin, 29-227aa, Human, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
PRL; Prolactin; Lactogenic hormone; Luteotropic hormone; Mammotropin; OTTHuMP00000017727; Prolactin precursor.
Sequence
MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC
Host
E Coli
Form/Format
Liquid. In phosphate buffer saline (pH 7.4)
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at +2 to +8 degrees C for 1 week.
For long term storage, aliquot and store at -20 degrees C to -80 degrees C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23 kDa (200 aa), confirmed by MALDI-TOF.
OMIM
V00566 mRNA
3D Structure
ModBase 3D Structure for P01236
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 0.5 ng/ml.

Product Categories/Family for PRL active protein

Applications Tested/Suitable for PRL active protein
SDS-PAGE, Bioactivity

SDS-Page of PRL active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
PRL Active Protein – Cat# MBS203093

Biological Activity of PRL active protein

Human Prolactin in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range is less than or equal to 0.5ng/ml.
PRL Active Protein, Biological Activity – Cat# MBS203093

Product References and Citations for PRL active protein

Ben-jonathan N., et al. (1996) Endocr Rev. 17 (6):639-69.; Cooke NE., et al. (1981) J. Biol. Chem. 256, 4007-4016.; Goffin V., et al. (1999) Mol Cell Endocrinol. 151, 79-87.;

Other Notes

Small volumes of PRL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRL purchase

MBS203093 is an active protein targeting PRL. Also known as Prolactin, 29-227aa, Human, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PRL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
PRL
NCBI Official Synonym Symbols
GHA1
UniProt Protein Name
Prolactin
Protein Family
UniProt Gene Name
PRL
UniProt Synonym Gene Names
PRL
NCBI Protein Information
prolactin

NCBI Summary for PRL

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
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UniProt Comments for PRL

Prolactin acts primarily on the mammary gland by promoting lactation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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