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GrpE Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203115

GrpE,co-chaperone of E Coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GrpE, Recombinant Protein
Full Product Name
GrpE, 1-197aa, E Coli, E Coli
Product Gene Name
Product Synonym Names
HSP-70 cofactor; Heat shock protein B25.3; HSP24; GrpE; HSP 70 cofactor.
Sequence
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Host
E Coli
Form/Format
Liquid. In 20mM Tris (pH 8.0), 100mM NaCl
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
21.8kDa (197aa), confirmed by MALDI-TOF.
3D Structure
ModBase 3D Structure for P09372
Antigen Species
E Coli

Product Categories/Family for GrpE recombinant protein

Applications Tested/Suitable for GrpE recombinant protein
SDS-PAGE

Product References and Citations for GrpE recombinant protein

Lipinska B, King J, Ang D, Georgopoulos C (1988). Nucleic Acids Res.16: 545-7562; Liberek K., Marszzalek J., Ang D, Georgopoulos C., Zylicz M. (1991). Proc. Natl. Acad.Sci. u.S.A. 88: 2874-2878.;

Other Notes

Small volumes of GrpE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GrpE purchase

MBS203115 is a recombinant protein targeting GrpE. Also known as GrpE, 1-197aa, E Coli, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GrpE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
heat shock protein
NCBI Official Symbol
grpE
NCBI Official Synonym Symbols
ECK2610; JW2594
UniProt Protein Name
Protein GrpE
UniProt Synonym Protein Names
HSP-70 cofactor; HSP24; Heat shock protein B25.3
Protein Family
UniProt Gene Name
grpE
NCBI Protein Information
heat shock protein

NCBI Summary for GrpE

HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10416]. Review: [More information is available at EcoCyc: EG10416].

UniProt Comments for GrpE

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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