Cyclophilin H (PPIH) Active Protein

Name: PPIH Active Protein; Cyclophilin H; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203201
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203201

Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of… More details

$245 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cyclophilin H (PPIH), Active Protein

Full Product Name

Cyclophilin H (PPIH), 1-177aa, Human,E Coli (Bioactivity Validated)

Product Gene Name

PPIH active protein

Product Synonym Names

Peptidylprolyl isomerase H; Peptidylprolyl isomerase H; PPIH; CYPH; CYP20; SnuCyp-20; SnuCyp-20; PPIase H; Rotamase H; Cyclophilin H; Peptidylprolyl isomerase H CypH; EC 5.2.1.8; MGC5016; Small nuclear ribonucleoprotein particle-specific cyclophilin H; uSACYP; usnRNP associated cyclophilin SnuCyp20

Sequence

MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

Host

E Coli

Form/Format

Liquid in phosphate buffered saline (pH 7.4), 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

19.2 kDa (177 aa), confirmed by MALDI-TOF.

OMIM

AF016371 mRNA

3D Structure

ModBase 3D Structure for O43447

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity

Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Assay

1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
2. Add 10ul of recombinant Cyclophikin H (PPIH) protein with 1ug in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25C.

Product Categories/Family for PPIH active protein

Cell Cycle

Applications Tested/Suitable for PPIH active protein

SDS-PAGE

Product References and Citations for PPIH active protein

Reidt u., et al. (2000) J Biol Chem. 275 (11):7439-42.; Horowitz DS., et al. (2002) EMBO. 21 (3):470-80.;

Other Notes

Small volumes of PPIH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIH purchase

MBS203201 is an active protein targeting PPIH. Also known as Cyclophilin H (PPIH), 1-177aa, Human,E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIH. It may not necessarily be applicable to this product.

NCBI GI #

5454154

NCBI GeneID

10465

NCBI Accession #

NP_006338

UniProt Primary Accession #

O43447

UniProt Secondary Accession #

A6NNE7

UniProt Related Accession #

O43447

GenBank Nucleotide #

NM_006347.3

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase H isoform 1

NCBI Official Synonym Full Names

peptidylprolyl isomerase H

NCBI Official Symbol

PPIH

NCBI Official Synonym Symbols

CYPH; CYP-20; USA-CYP; SnuCyp-20

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase H

UniProt Synonym Protein Names

Rotamase H; Small nuclear ribonucleoprotein particle-specific cyclophilin H; CypH; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

PPIH

UniProt Synonym Gene Names

CYP20; CYPH; PPIase H; CypH; USA-CYP

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase H

NCBI Summary for PPIH

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]

UniProt Comments for PPIH

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.

Research Articles on PPIH

1. Free and complexed cyclophilin H have virtually identical conformations suggesting that the U4/U6-60K binding site is pre-shaped and the peptidyl-prolyl-cis/trans isomerase activity is unaffected by complex formation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PPIH active protein

Reference Product	PubMed Publications
PRPF4 active protein	>1 publications with PPIH and PRPF4

Pathways associated with PPIH active protein

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies	Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram
Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies	Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram

Diseases associated with PPIH active protein

Disease Name	Pubmed Publications
Hypertension Antibodies	>7 publications with PPIH and Hypertension
Hemorrhage Antibodies	>1 publications with PPIH and Hemorrhage
Cardiovascular Diseases Antibodies	>1 publications with PPIH and Cardiovascular Diseases

Organs/Tissues associated with PPIH active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>9 publications with PPIH and Blood
Vascular Antibodies	>6 publications with PPIH and Vascular
Uterus Antibodies	>5 publications with PPIH and Uterus
Umbilical Cord Antibodies	>3 publications with PPIH and Umbilical Cord
Kidney Antibodies	>1 publications with PPIH and Kidney
Placenta Antibodies	>1 publications with PPIH and Placenta
Intestine Antibodies	>1 publications with PPIH and Intestine