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Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032498
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MBS2032498 is an antibody targeting SMUG1. Also known as Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1), Polyclonal Antibody
Full Product Name
Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Polyclonal Antibody
Product Gene Name
anti-SMUG1 antibody
Product Synonym Names
FDG; HMUDG; UNG3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against SMUG1. It has been selected for its ability to recognize SMUG1 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: SMUG1 (MBS2032498)
Immunogen: SMUG1 (MBS2033573)
Matching Pairs
Unconjugated Antibody: SMUG1 (MBS2032498)
APC-CY7 Conjugated Antibody: SMUG1 (MBS2079371)
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Molecular Weight
19,627 Da
OMIM
AF125182 mRNA
3D Structure
ModBase 3D Structure for Q53HV7
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
SMUG1 (Met1~Lys270)
Quality Control
Content: The quality control contains recombinant SMUG1 (Met1~Lys270) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079373
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-SMUG1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-SMUG1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-SMUG1 antibody

Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SMUG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-SMUG1 Polyclonal Antibody – Cat# MBS2032498

Western Blot (WB) of anti-SMUG1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-SMUG1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2032498

Immunohistochemistry (IHC) of anti-SMUG1 antibody

DAB staining on fromalin fixed paraffin- embedded liver tissue)
Rabbit Anti-SMUG1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032498

Other Notes

Small volumes of anti-SMUG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMUG1. It may not necessarily be applicable to this product.

NCBI GI #
344925869
NCBI GeneID
23583
NCBI Accession #
NP_001230716.1
UniProt Primary Accession #
Q53HV7
UniProt Secondary Accession #
O95862; Q0D2M0; Q8NB71; Q9BWC8; A8K2K9
UniProt Related Accession #
Q53HV7
GenBank Nucleotide #
NM_001243787.1
NCBI Official Full Name
single-strand selective monofunctional uracil DNA glycosylase isoform 1
NCBI Official Synonym Full Names
single-strand-selective monofunctional uracil-DNA glycosylase 1
NCBI Official Symbol
SMUG1
NCBI Official Synonym Symbols
FDG; UNG3; HMUDG
UniProt Protein Name
Single-strand selective monofunctional uracil DNA glycosylase
Protein Family
Single-strand selective monofunctional uracil DNA glycosylase
UniProt Gene Name
SMUG1
NCBI Protein Information
single-strand selective monofunctional uracil DNA glycosylase

NCBI Summary for SMUG1

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

UniProt Comments for SMUG1

SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Hydrolase

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleolus; nucleoplasm

Molecular Function: DNA binding; DNA N-glycosylase activity; oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; depyrimidination
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Research Articles on SMUG1

1. Our study showed that c.-31A/G-SMUG1 genotypes/alleles do not have any association with the occurrence or severity of advanced type age-related macular degeneration (AMD). There was no interaction of CRP levels and SMUG1 genotypes in AMD susceptibility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SMUG1 antibody

 Products by Pathway  Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base-Excision Repair, AP Site Formation Pathway antibodies  Base-Excision Repair, AP Site Formation Pathway Diagram
 Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 Cleavage Of The Damaged Pyrimidine Pathway antibodies  Cleavage Of The Damaged Pyrimidine Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Depyrimidination Pathway antibodies  Depyrimidination Pathway Diagram
 Displacement Of DNA Glycosylase By APE1 Pathway antibodies  Displacement Of DNA Glycosylase By APE1 Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram

Diseases associated with anti-SMUG1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >9 publications with SMUG1 and Neoplasms
 Carcinoma Antibodies  >3 publications with SMUG1 and Carcinoma
 Genetic Predisposition to Disease Antibodies  >2 publications with SMUG1 and Genetic Predisposition to Disease
 Hyperplasia Antibodies  >2 publications with SMUG1 and Hyperplasia
 Breast Neoplasms Antibodies  >1 publications with SMUG1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >1 publications with SMUG1 and Nervous System Diseases
 Inflammation Antibodies  >1 publications with SMUG1 and Inflammation

Organs/Tissues associated with anti-SMUG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with SMUG1 and Liver
 Lung Antibodies  >1 publications with SMUG1 and Lung
 Muscle Antibodies  >1 publications with SMUG1 and Muscle
 Lymph Node Antibodies  >1 publications with SMUG1 and Lymph Node
 Skin Antibodies  >1 publications with SMUG1 and Skin
 Brain Antibodies  >1 publications with SMUG1 and Brain
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