3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

GNMT Recombinant Protein

Name: GNMT Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203259
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203259

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GNMT, Recombinant Protein

Full Product Name

GNMT, 1-295aa, Human,His tag, E Coli

Product Gene Name

GNMT recombinant protein

Product Synonym Names

Glycine N-methyltransferase; GNMT; Glycine N-methyltransferase; Glycine N-methyltransferase EC 2.1.1.20; Glycine N methyltransferase.

Sequence

MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD

Host

E Coli

Form/Format

Liquid in 20mM Tris pH 8.0, 20% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Molecular Weight

34.9kDa (315aa), confirmed by MALDI-TOF.

OMIM

AF101477 mRNA

3D Structure

ModBase 3D Structure for Q14749

Tag Information

His-tag

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Domain

1-295aa

Product Categories/Family for GNMT recombinant protein

Enzymes & Proteases

Applications Tested/Suitable for GNMT recombinant protein

SDS-PAGE

Product References and Citations for GNMT recombinant protein

Liao YJ., et al. (2009) Int J Cancer.124 (4):816-26.; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23 (9):1384-9.;

Other Notes

Small volumes of GNMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNMT purchase

MBS203259 is a recombinant protein targeting GNMT. Also known as GNMT, 1-295aa, Human,His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GNMT. It may not necessarily be applicable to this product.

NCBI GI #

9506741

NCBI GeneID

27232

NCBI Accession #

NP_061833

UniProt Primary Accession #

Q14749

UniProt Secondary Accession #

Q5T8W2; Q9NNZ1; Q9NS24

UniProt Related Accession #

Q14749

GenBank Nucleotide #

NM_018960.5

NCBI Official Full Name

glycine N-methyltransferase isoform 1

NCBI Official Synonym Full Names

glycine N-methyltransferase

NCBI Official Symbol

GNMT

NCBI Official Synonym Symbols

HEL-S-182mP

UniProt Protein Name

Glycine N-methyltransferase

Protein Family

Glycine N-methyltransferase

UniProt Gene Name

GNMT

NCBI Protein Information

glycine N-methyltransferase

NCBI Summary for GNMT

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016]

UniProt Comments for GNMT

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Research Articles on GNMT

1. Elevated GNMT protein expression is associated with pathogenesis of hepatocellular carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GNMT recombinant protein

Reference Product	PubMed Publications
SHMT1 recombinant protein	>1 publications with GNMT and SHMT1

Pathways associated with GNMT recombinant protein

Products by Pathway	Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glyoxylate Metabolism And Glycine Degradation Pathway antibodies	Glyoxylate Metabolism And Glycine Degradation Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Metabolism Of Ingested SeMet, Sec, MeSec Into H2Se Pathway antibodies	Metabolism Of Ingested SeMet, Sec, MeSec Into H2Se Pathway Diagram
Selenoamino Acid Metabolism Pathway antibodies	Selenoamino Acid Metabolism Pathway Diagram

Diseases associated with GNMT recombinant protein

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>55 publications with GNMT and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>41 publications with GNMT and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>33 publications with GNMT and Liver Neoplasms
Adenocarcinoma Antibodies	>29 publications with GNMT and Adenocarcinoma
Disease Models, Animal Antibodies	>25 publications with GNMT and Disease Models, Animal
Fatty Liver Antibodies	>19 publications with GNMT and Fatty Liver
Hepatitis Antibodies	>13 publications with GNMT and Hepatitis
Prostatic Neoplasms Antibodies	>11 publications with GNMT and Prostatic Neoplasms
Hyperhomocysteinemia Antibodies	>9 publications with GNMT and Hyperhomocysteinemia
Hepatitis, Chronic Antibodies	>9 publications with GNMT and Hepatitis, Chronic

Organs/Tissues associated with GNMT recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>130 publications with GNMT and Liver
Blood Antibodies	>33 publications with GNMT and Blood
Prostate Antibodies	>13 publications with GNMT and Prostate
Kidney Antibodies	>7 publications with GNMT and Kidney
Pancreas Antibodies	>6 publications with GNMT and Pancreas
Lung Antibodies	>3 publications with GNMT and Lung
Testis Antibodies	>1 publications with GNMT and Testis