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MGMT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203260

O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of… More details

$245 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MGMT, Recombinant Protein
Full Product Name
MGMT, 1-207aa, Human, His tag, E Coli
Product Gene Name
MGMT recombinant protein
Product Synonym Names
O-6-methylguanine-DNA methyltransferase; MGMT; EC 2.1.1.63; O-6-methylguanine-DNA-alkyltransferase; O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; MGC107020; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase.
Sequence
MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
OMIM
X54228 mRNA
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for MGMT recombinant protein

Enzymes & Proteases
Applications Tested/Suitable for MGMT recombinant protein
SDS-PAGE

SDS-Page of MGMT recombinant protein

MGMT Recombinant Protein – Cat# MBS203260

Product References and Citations for MGMT recombinant protein

Matsukura S., et al. (2001) Annals of Surgical Oncology, 8 (10):807--816; Esteller M.,et al. (2002) J Natl Cancer Inst. 2, 26-32.;

Other Notes

Small volumes of MGMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MGMT purchase

MBS203260 is a recombinant protein targeting MGMT. Also known as MGMT, 1-207aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MGMT. It may not necessarily be applicable to this product.

NCBI GI #
197304670
NCBI GeneID
4255
NCBI Accession #
NP_002403
UniProt Secondary Accession #
Q5VY78
UniProt Related Accession #
P16455
GenBank Nucleotide #
NM_002412.4
NCBI Official Full Name
methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family
Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
MGMT
UniProt Synonym Gene Names
MGMT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase

NCBI Summary for MGMT

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
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UniProt Comments for MGMT

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
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Research Articles on MGMT

1. Findings show the cell-context dependent effects of PRIMA-1MET irrespective of p53 status and suggest the role of O6-methylguanine-DNA methyltransferase (MGMT) as a potential molecular target of PRIMA-1MET in MGMT-positive glioblastoma stem cell (GSCs).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MGMT recombinant protein

 Reference Product  PubMed Publications
 CDKN2A recombinant protein  >430 publications with MGMT and CDKN2A
 MLH1 recombinant protein  >255 publications with MGMT and MLH1
 TP53 recombinant protein  >152 publications with MGMT and TP53
 PTEN recombinant protein  >118 publications with MGMT and PTEN
 KRAS recombinant protein  >102 publications with MGMT and KRAS
 RASSF1 recombinant protein  >91 publications with MGMT and RASSF1
 MSH2 recombinant protein  >73 publications with MGMT and MSH2
 DAPK1 recombinant protein  >50 publications with MGMT and DAPK1
 HRAS recombinant protein  >1 publications with MGMT and HRAS

Pathways associated with MGMT recombinant protein

 Products by Pathway  Pathway Diagram
 DNA Damage Reversal Pathway antibodies  DNA Damage Reversal Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 MGMT-mediated DNA Damage Reversal Pathway antibodies  MGMT-mediated DNA Damage Reversal Pathway Diagram

Diseases associated with MGMT recombinant protein

 Disease Name  Pubmed Publications
 Glioma Antibodies  >1417 publications with MGMT and Glioma
 Nervous System Diseases Antibodies  >1213 publications with MGMT and Nervous System Diseases
 Brain Neoplasms Antibodies  >1183 publications with MGMT and Brain Neoplasms
 Glioblastoma Antibodies  >1148 publications with MGMT and Glioblastoma
 Carcinoma Antibodies  >537 publications with MGMT and Carcinoma
 Recurrence Antibodies  >295 publications with MGMT and Recurrence
 Adenocarcinoma Antibodies  >202 publications with MGMT and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >178 publications with MGMT and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >172 publications with MGMT and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >139 publications with MGMT and Carcinoma, Squamous Cell

Organs/Tissues associated with MGMT recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1308 publications with MGMT and Brain
 Blood Antibodies  >324 publications with MGMT and Blood
 Lung Antibodies  >226 publications with MGMT and Lung
 Liver Antibodies  >136 publications with MGMT and Liver
 Bone Antibodies  >122 publications with MGMT and Bone
 Stomach Antibodies  >84 publications with MGMT and Stomach
 Intestine Antibodies  >68 publications with MGMT and Intestine
 Vascular Antibodies  >68 publications with MGMT and Vascular
 Lymph Antibodies  >63 publications with MGMT and Lymph
 Skin Antibodies  >57 publications with MGMT and Skin
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