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Methionine Sulfoxide Reductase A (MSRA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032637
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MBS2032637 is an antibody targeting MSRA. Also known as Methionine Sulfoxide Reductase A (MSRA) Polyclonal Antibody. Reactive in Human, Rat, Pig. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Methionine Sulfoxide Reductase A (MSRA), Polyclonal Antibody
Full Product Name
Methionine Sulfoxide Reductase A (MSRA) Polyclonal Antibody
Product Gene Name
Product Synonym Names
PMSR; Peptide Methionine Sulfoxide Reductase; Protein-methionine-S-oxide reductase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Pig
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against MSRA. It has been selected for its ability to recognize MSRA in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: MSRA (MBS2032637)
Immunogen: MSRA (MBS2033401)
Matching Pairs
Unconjugated Antibody: MSRA (MBS2032637)
APC-CY7 Conjugated Antibody: MSRA (MBS2067258)
Molecular Weight
23,627 Da
OMIM
AJ242973 mRNA
3D Structure
ModBase 3D Structure for Q9UJ68
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Rat, Pig
Organism Species
Human
Fragment
MSRA (Ala27~Lys235)
Quality Control
Content: The quality control contains recombinant MSRA (Ala27~Lys235) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067260
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-MSRA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-MSRA antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-MSRA antibody

Western Blot: Sample: Human HT1080 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human MSRA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-MSRA Polyclonal Antibody – Cat# MBS2032637

Western Blot (WB) of anti-MSRA antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-MSRA Polyclonal Antibody, Western Blot (WB) – Cat# MBS2032637

Immunohistochemistry (IHC) of anti-MSRA antibody

DAB staining on IHC-P. Samples: Human Tissue)
Rabbit Anti-MSRA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032637

Other Notes

Small volumes of anti-MSRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSRA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitochondrial peptide methionine sulfoxide reductase isoform b
NCBI Official Synonym Full Names
methionine sulfoxide reductase A
NCBI Official Symbol
MSRA
NCBI Official Synonym Symbols
PMSR
UniProt Protein Name
Mitochondrial peptide methionine sulfoxide reductase
UniProt Synonym Protein Names
Peptide-methionine (S)-S-oxide reductase; Peptide Met(O) reductase; Protein-methionine-S-oxide reductase; PMSR
UniProt Gene Name
MSRA
UniProt Synonym Gene Names
Peptide Met(O) reductase; PMSR
NCBI Protein Information
mitochondrial peptide methionine sulfoxide reductase

NCBI Summary for MSRA

This gene encodes a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein functions in the repair of oxidatively damaged proteins to restore biological activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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UniProt Comments for MSRA

MSRA: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. Belongs to the MsrA Met sulfoxide reductase family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 1.8.4.11; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: actin cytoskeleton; cytosol; membrane; mitochondrion; nucleoplasm

Molecular Function: peptide-methionine (S)-S-oxide reductase activity

Biological Process: cellular protein modification process; methionine metabolic process; protein repair; response to oxidative stress
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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