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Mitochondrial Ribosomal Protein L1 (MRPL1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032687
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MBS2032687 is an antibody targeting MRPL1. Also known as Mitochondrial Ribosomal Protein L1 (MRPL1) Polyclonal Antibody. Reactive in Mouse, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mitochondrial Ribosomal Protein L1 (MRPL1), Polyclonal Antibody
Full Product Name
Mitochondrial Ribosomal Protein L1 (MRPL1) Polyclonal Antibody
Product Gene Name
anti-MRPL1 antibody
Product Synonym Names
MRP-L1; BM022; L1mt; 39S Ribosomal Protein L1, Mitochondrial
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
0.57mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against MRPL1. It has been selected for its ability to recognize MRPL1 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: MRPL1 (MBS2032687)
Immunogen: MRPL1 (MBS2033077)
Matching Pairs
Unconjugated Antibody: MRPL1 (MBS2032687)
APC-CY7 Conjugated Antibody: MRPL1 (MBS2067235)
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Molecular Weight
37,597 Da
3D Structure
ModBase 3D Structure for Q99N96
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
MRPL1 (Glu143~Ala336)
Quality Control
Content: The quality control contains recombinant MRPL1 (Glu143~Ala336) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067237
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-MRPL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-MRPL1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-MRPL1 antibody

Western Blot: Sample: Recombinant MRPL1, Mouse.
Rabbit Anti-MRPL1 Polyclonal Antibody – Cat# MBS2032687

Immunohistochemistry (IHC) of anti-MRPL1 antibody

DAB staining on IHC-P; Samples: Mouse Stomach Tissue.
Rabbit Anti-MRPL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032687

Immunohistochemistry (IHC) of anti-MRPL1 antibody

DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Rabbit Anti-MRPL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032687

Other Notes

Small volumes of anti-MRPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MRPL1. It may not necessarily be applicable to this product.

NCBI GI #
84875528
NCBI GeneID
94061
NCBI Accession #
NP_444388.2
UniProt Primary Accession #
Q99N96
UniProt Secondary Accession #
Q3UQP5; Q8K351; Q9CWW4
UniProt Related Accession #
Q99N96
GenBank Nucleotide #
NM_053158.3
NCBI Official Full Name
39S ribosomal protein L1, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L1
NCBI Official Symbol
Mrpl1
NCBI Official Synonym Symbols
L1mt; AI462572; 2410002L03Rik; 5830418D04Rik
UniProt Protein Name
39S ribosomal protein L1, mitochondrial
Protein Family
39S ribosomal protein
UniProt Gene Name
Mrpl1
UniProt Synonym Gene Names
L1mt; MRP-L1
NCBI Protein Information
39S ribosomal protein L1, mitochondrial

UniProt Comments for MRPL1

MRPL1: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; RNA-binding

Chromosomal Location of Human Ortholog: 5|5 E3

Cellular Component: intracellular ribonucleoprotein complex; large ribosomal subunit; mitochondrial large ribosomal subunit; mitochondrion; ribosome

Molecular Function: RNA binding; structural constituent of ribosome

Biological Process: cleavage involved in rRNA processing; maturation of LSU-rRNA; rRNA pseudouridine synthesis; snRNA pseudouridine synthesis; translation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MRPL1 antibody

 Products by Pathway  Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram

Diseases associated with anti-MRPL1 antibody

 Disease Name  Pubmed Publications
 Abnormalities, Drug-Induced Antibodies  >1 publications with MRPL1 and Abnormalities, Drug-Induced
 Congenital Abnormalities Antibodies  >1 publications with MRPL1 and Congenital Abnormalities
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