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Acid Phosphatase 1 (ACP1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203269

Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein… More details

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Product Overview

Online Datasheet in PDF
Product Name
Acid phosphatase 1 (ACP1), Active Protein
Full Product Name
Acid phosphatase 1,1-158aa, Human, His tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
Acid phosphatase 1 soluble isoform b; Acid phosphatase 1; soluble isoform b; ACP1; HAAP; LMW-PTP; Red cell acid phosphatase 1; Adipocyte acid phosphatase; Acid phosphatase 1; soluble isoform b Acid phosphatase 1 soluble; Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase; Low molecular weight phosphotyrosine protein phosphatase; Acid phosphatase of erythrocyte; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1.
Sequence
MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
Host
E Coli
Form/Format
Liquid. In 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2-8 degree C for 1 week.
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
20.1 kDa (178 aa), confirmed by MALDI-TOF.
OMIM
M83653 mRNA
3D Structure
ModBase 3D Structure for P24666
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Domain
1-158aa
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 15,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Categories/Family for ACP1 active protein

Applications Tested/Suitable for ACP1 active protein
SDS-PAGE, Enzyme Activity

SDS-Page of ACP1 active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
ACP1 Active Protein – Cat# MBS203269

Product References and Citations for ACP1 active protein

De Lorenzo A., et al. (2009) Metabolism.58 (3):351-4; Banci M., et al. (2009) Cardiology.113 (4):236-242.;

Other Notes

Small volumes of ACP1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACP1 purchase

MBS203269 is an active protein targeting ACP1. Also known as Acid phosphatase 1,1-158aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ACP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
low molecular weight phosphotyrosine protein phosphatase isoform b
NCBI Official Synonym Full Names
acid phosphatase 1
NCBI Official Symbol
ACP1
NCBI Official Synonym Symbols
HAAP; LMWPTP; LMW-PTP
UniProt Protein Name
Low molecular weight phosphotyrosine protein phosphatase
UniProt Synonym Protein Names
Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2); Red cell acid phosphatase 1
Protein Family
UniProt Gene Name
ACP1
UniProt Synonym Gene Names
LMW-PTP; LMW-PTPase
NCBI Protein Information
low molecular weight phosphotyrosine protein phosphatase

NCBI Summary for ACP1

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]
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UniProt Comments for ACP1

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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