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General Transcription Factor IIA, Polypeptide 1 (GTF2A1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032706
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MBS2032706 is an antibody targeting GTF2A1. Also known as General Transcription Factor IIA, Polypeptide 1 (GTF2A1) Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
General Transcription Factor IIA, Polypeptide 1 (GTF2A1), Polyclonal Antibody
Full Product Name
General Transcription Factor IIA, Polypeptide 1 (GTF2A1) Polyclonal Antibody
Product Gene Name
anti-GTF2A1 antibody
Product Synonym Names
GTF2-A1; TF2A1; TFIIA; Transcription Initiation Factor IIA Subunit 1; Transcription initiation factor TFIIA 42 kDa subunit
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against GTF2A1. It has been selected for its ability to recognize GTF2A1 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: GTF2A1 (MBS2032706)
Immunogen: GTF2A1 (MBS2033559)
Matching Pairs
Unconjugated Antibody: GTF2A1 (MBS2032706)
APC-CY7 Conjugated Antibody: GTF2A1 (MBS2066471)
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Molecular Weight
37,152 Da
OMIM
X75383 mRNA
3D Structure
ModBase 3D Structure for P52655
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
GTF2A1 (Ala2~Asp274)
Quality Control
Content: The quality control contains recombinant GTF2A1 (Ala2~Asp274) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066473
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-GTF2A1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-GTF2A1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-GTF2A1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-GTF2A1 Polyclonal Antibody – Cat# MBS2032706

Immunohistochemistry (IHC) of anti-GTF2A1 antibody

DABstainingonIHC-P.Samples:HumanTissue)
Rabbit Anti-GTF2A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032706

Other Notes

Small volumes of anti-GTF2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2A1. It may not necessarily be applicable to this product.

NCBI GI #
525313614
NCBI GeneID
2957
NCBI Accession #
NP_001265869.1
UniProt Primary Accession #
P52655
UniProt Secondary Accession #
Q3KNQ9
UniProt Related Accession #
P52655
GenBank Nucleotide #
NM_001278940.1
NCBI Official Full Name
transcription initiation factor IIA subunit 1 isoform 3
NCBI Official Synonym Full Names
general transcription factor IIA subunit 1
NCBI Official Symbol
GTF2A1
NCBI Official Synonym Symbols
TF2A1; TFIIA; TFIIAL; TFIIA-42
UniProt Protein Name
Transcription initiation factor IIA subunit 1
UniProt Synonym Protein Names
General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Protein Family
Transcription initiation factor
UniProt Gene Name
GTF2A1
UniProt Synonym Gene Names
TF2A1; TFIIA-42
NCBI Protein Information
transcription initiation factor IIA subunit 1

NCBI Summary for GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]
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UniProt Comments for GTF2A1

GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: cytosol; nucleoplasm; transcription factor TFIIA complex; transcription factor TFIID complex

Molecular Function: DNA binding; protein binding; protein heterodimerization activity; TATA-binding protein binding; transcription coactivator activity; transcription factor binding

Biological Process: regulation of transcription, DNA-templated; snRNA transcription from RNA polymerase II promoter; transcription elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
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Research Articles on GTF2A1

1. In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GTF2A1 antibody

 Reference Product  PubMed Publications
 GTF2A2 antibody  >1 publications with GTF2A1 and GTF2A2
 TBP antibody  >1 publications with GTF2A1 and TBP

Pathways associated with anti-GTF2A1 antibody

 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 HIV Transcription Initiation Pathway antibodies  HIV Transcription Initiation Pathway Diagram
 Late Phase Of HIV Life Cycle Pathway antibodies  Late Phase Of HIV Life Cycle Pathway Diagram
 RNA Polymerase II HIV Promoter Escape Pathway antibodies  RNA Polymerase II HIV Promoter Escape Pathway Diagram
 RNA Polymerase II Pre-transcription Events Pathway antibodies  RNA Polymerase II Pre-transcription Events Pathway Diagram

Diseases associated with anti-GTF2A1 antibody

 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >1 publications with GTF2A1 and Ovarian Neoplasms

Organs/Tissues associated with anti-GTF2A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >1 publications with GTF2A1 and Stomach
 Testis Antibodies  >1 publications with GTF2A1 and Testis
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