Baculovirus DKK-1 Recombinant Protein – Cat# MBS203274

DKK-1 Baculovirus Recombinant Protein

Name: DKK-1 Recombinant Protein; Baculovirus; MyBioSource
Brand: MyBioSource
SKU: MBS203274
Price: 300 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203274

Dickkopf-related protein 1, also known as Dkk-1, is a member of the Dkk protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling… More details

$300 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Dkk-1, Recombinant Protein

Full Product Name

Dkk-1, 32-266aa, Human, His tag, Baculovirus

Product Gene Name

Dkk-1 recombinant protein

Product Synonym Names

Dickkopf-related protein 1; Dickkopf-1; hDkk-1; SK; Dickkopf-related protein 1 Dickkopf 1; Dickkopf 1 like; Dickkopf homolog 1; Dickkopf related protein 1; DKK 1; hDkk 1; DKK1

Sequence

ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH

Host

Baculovirus

Form/Format

Liquid. In phosphate buffered saline (pH 7.4) containing 50% glycerol, 2mM DTT, 2mM EDTA, 0.1mM PMSF

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

27.8kDa (253aa) 28-40kDa (SDS-PAGE under reducing conditions)

OMIM

AF127563 mRNA

3D Structure

ModBase 3D Structure for O94907

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for Dkk-1 recombinant protein

Signal Transduction

Applications Tested/Suitable for Dkk-1 recombinant protein

SDS-PAGE

Product References and Citations for Dkk-1 recombinant protein

Krupnik, V.E. et al. (1999) Gene 238:301-13 ; Nusse, R. et al. (2001) Nature 411:255-6 ; Mao, J. et al. (2001) Mol. Cell 7:801-9;

Other Notes

Small volumes of Dkk-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dkk-1 purchase

MBS203274 is a recombinant protein targeting Dkk-1. Also known as Dkk-1, 32-266aa, Human, His tag, Baculovirus. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Dkk-1. It may not necessarily be applicable to this product.

NCBI GI #

7110719

NCBI GeneID

22943

NCBI Accession #

NP_036374

UniProt Primary Accession #

O94907

UniProt Secondary Accession #

B2RC19

UniProt Related Accession #

O94907

GenBank Nucleotide #

NM_012242.3

NCBI Official Full Name

dickkopf-related protein 1

NCBI Official Synonym Full Names

dickkopf WNT signaling pathway inhibitor 1

NCBI Official Symbol

DKK1

NCBI Official Synonym Symbols

SK; DKK-1

UniProt Protein Name

Dickkopf-related protein 1

UniProt Synonym Protein Names

Protein Family

Dickkopf-related protein

UniProt Gene Name

DKK1

UniProt Synonym Gene Names

Dickkopf-1; Dkk-1; hDkk-1

NCBI Protein Information

dickkopf-related protein 1

NCBI Summary for Dkk-1

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

UniProt Comments for Dkk-1

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity ().

Research Articles on Dkk-1

1. Sclerostin But Not Dickkopf-1 has roles in increasing prevalence of osteoporotic fracture and lower bone mineral density in postmenopausal Korean women

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Dkk-1 recombinant protein

Reference Product	PubMed Publications
WNT3A recombinant protein	>163 publications with Dkk-1 and WNT3A
LRP5 recombinant protein	>147 publications with Dkk-1 and LRP5
LRP6 recombinant protein	>129 publications with Dkk-1 and LRP6
CTNNB1 recombinant protein	>125 publications with Dkk-1 and CTNNB1
MYC recombinant protein	>75 publications with Dkk-1 and MYC
WNT1 recombinant protein	>57 publications with Dkk-1 and WNT1
KREMEN1 recombinant protein	>22 publications with Dkk-1 and KREMEN1
KREMEN2 recombinant protein	>15 publications with Dkk-1 and KREMEN2
WNT8A recombinant protein	>14 publications with Dkk-1 and WNT8A
FZD1 recombinant protein	>14 publications with Dkk-1 and FZD1

Pathways associated with Dkk-1 recombinant protein

Products by Pathway	Pathway Diagram
BMP2-WNT4-FOXO1 Pathway In Human Primary Endometrial Stromal Cell Differentiation antibodies	BMP2-WNT4-FOXO1 Pathway In Human Primary Endometrial Stromal Cell Differentiation Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Differentiation Pathway antibodies	Differentiation Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Of Signal Transduction Pathway antibodies	Diseases Of Signal Transduction Pathway Diagram
Endoderm Differentiation Pathway antibodies	Endoderm Differentiation Pathway Diagram
Mesodermal Commitment Pathway antibodies	Mesodermal Commitment Pathway Diagram
Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies	Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway antibodies	Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway Diagram

Diseases associated with Dkk-1 recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>122 publications with Dkk-1 and Cardiovascular Diseases
Inflammation Antibodies	>90 publications with Dkk-1 and Inflammation
Liver Diseases Antibodies	>63 publications with Dkk-1 and Liver Diseases
Breast Neoplasms Antibodies	>59 publications with Dkk-1 and Breast Neoplasms
Fractures, Bone Antibodies	>54 publications with Dkk-1 and Fractures, Bone
Brain Diseases Antibodies	>52 publications with Dkk-1 and Brain Diseases
Necrosis Antibodies	>52 publications with Dkk-1 and Necrosis
Neoplasm Metastasis Antibodies	>51 publications with Dkk-1 and Neoplasm Metastasis
Kidney Diseases Antibodies	>50 publications with Dkk-1 and Kidney Diseases
Fibrosis Antibodies	>48 publications with Dkk-1 and Fibrosis

Organs/Tissues associated with Dkk-1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>695 publications with Dkk-1 and Bone
Bone Marrow Antibodies	>133 publications with Dkk-1 and Bone Marrow
Embryonic Tissue Antibodies	>119 publications with Dkk-1 and Embryonic Tissue
Connective Tissue Antibodies	>116 publications with Dkk-1 and Connective Tissue
Brain Antibodies	>107 publications with Dkk-1 and Brain
Vascular Antibodies	>95 publications with Dkk-1 and Vascular
Liver Antibodies	>87 publications with Dkk-1 and Liver
Lung Antibodies	>74 publications with Dkk-1 and Lung
Muscle Antibodies	>72 publications with Dkk-1 and Muscle
Skin Antibodies	>66 publications with Dkk-1 and Skin