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Pyridoxamine-5'-Phosphate Oxidase (PNPO) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032877
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2032877 is an antibody targeting PNPO. Also known as Pyridoxamine-5'-Phosphate Oxidase (PNPO) Polyclonal Antibody. Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pyridoxamine-5'-Phosphate Oxidase (PNPO), Polyclonal Antibody
Full Product Name
Pyridoxamine-5'-Phosphate Oxidase (PNPO) Polyclonal Antibody
Product Gene Name
anti-PNPO antibody
Product Synonym Names
PDXPO; Pyridoxine 5'-Phosphate Oxidase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against PNPO. It has been selected for its ability to recognize PNPO in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: PNPO (MBS2032877)
Immunogen: PNPO (MBS2033530)
Matching Pairs
Unconjugated Antibody: PNPO (MBS2032877)
APC-CY7 Conjugated Antibody: PNPO (MBS2067855)
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Molecular Weight
30,114 Da
3D Structure
ModBase 3D Structure for Q91XF0
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
PNPO (Met1~Pro261)
Quality Control
Content: The quality control contains recombinant PNPO (Met1~Pro261) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067857
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PNPO antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-PNPO antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PNPO antibody

Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue; Lane3: Mouse Liver Tissue; Lane4: Mouse Kidney Tissue.
Rabbit Anti-PNPO Polyclonal Antibody – Cat# MBS2032877

Western Blot (WB) of anti-PNPO antibody

Western Blot: Sample: Recombinant PNPO, Mouse.
Rabbit Anti-PNPO Polyclonal Antibody, Western Blot (WB) – Cat# MBS2032877

Immunohistochemistry (IHC) of anti-PNPO antibody

DAB staining on IHC-P; Samples: Mouse Stomach Tissue.
Rabbit Anti-PNPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032877

Immunohistochemistry (IHC) of anti-PNPO antibody

DAB staining on IHC-P; Samples: Mouse Brain Tissue
Rabbit Anti-PNPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032877

Immunohistochemistry (IHC) of anti-PNPO antibody

DAB staining on IHC-P; Samples: Mouse Kidney Tissue
Rabbit Anti-PNPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032877

Other Notes

Small volumes of anti-PNPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PNPO. It may not necessarily be applicable to this product.

NCBI GI #
19527238
NCBI GeneID
103711
NCBI Accession #
NP_598782.1
UniProt Primary Accession #
Q91XF0
UniProt Secondary Accession #
Q3TP70; Q3U445; Q3U4X1
UniProt Related Accession #
Q91XF0
GenBank Nucleotide #
NM_134021.2
NCBI Official Full Name
pyridoxine-5'-phosphate oxidase
NCBI Official Synonym Full Names
pyridoxine 5'-phosphate oxidase
NCBI Official Symbol
Pnpo
NCBI Official Synonym Symbols
AI415282
UniProt Protein Name
Pyridoxine-5'-phosphate oxidase
UniProt Synonym Protein Names
Pyridoxamine-phosphate oxidase
Protein Family
Pyridoxine-5'-phosphate oxidase
UniProt Gene Name
Pnpo
NCBI Protein Information
pyridoxine-5'-phosphate oxidase

UniProt Comments for PNPO

PNPO: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Defects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency); also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine. Belongs to the pyridoxamine 5'-phosphate oxidase family.

Protein type: Cofactor and Vitamin Metabolism - vitamin B6; EC 1.4.3.5; Oxidoreductase

Chromosomal Location of Human Ortholog: 11|11 D

Cellular Component: cytosol; nucleoplasm

Molecular Function: cofactor binding; FMN binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-NH2 group of donors; protein homodimerization activity; pyridoxal phosphate binding; pyridoxamine-phosphate oxidase activity

Biological Process: pyridoxal phosphate biosynthetic process; pyridoxine biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PNPO antibody

 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with anti-PNPO antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >21 publications with PNPO and Brain Diseases
 Nervous System Diseases Antibodies  >21 publications with PNPO and Nervous System Diseases
 Pyridoxamine 5-Prime-Phosphate Oxidase Deficiency Antibodies  >4 publications with PNPO and Pyridoxamine 5-Prime-Phosphate Oxidase Deficiency
 Hypertension Antibodies  >3 publications with PNPO and Hypertension
 Liver Diseases Antibodies  >2 publications with PNPO and Liver Diseases
 Status Epilepticus Antibodies  >2 publications with PNPO and Status Epilepticus
 Infertility, Female Antibodies  >1 publications with PNPO and Infertility, Female
 Inflammation Antibodies  >1 publications with PNPO and Inflammation
 Liver Neoplasms Antibodies  >1 publications with PNPO and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with PNPO and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-PNPO antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >18 publications with PNPO and Brain
 Liver Antibodies  >5 publications with PNPO and Liver
 Heart Antibodies  >2 publications with PNPO and Heart
 Ovary Antibodies  >1 publications with PNPO and Ovary
 Eye Antibodies  >1 publications with PNPO and Eye
 Intestine Antibodies  >1 publications with PNPO and Intestine
 Lung Antibodies  >1 publications with PNPO and Lung
 Kidney Antibodies  >1 publications with PNPO and Kidney
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