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Carbonic Anhydrase 2 (CA2) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203326

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene… More details

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Product Overview

Online Datasheet in PDF
Product Name
Carbonic anhydrase 2 (CA2), Active Protein
Full Product Name
Carbonic anhydrase 2, 1-260aa, Human, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
Carbonic anhydrase 2; CA-II; CAC; CA 2; CA II; CA2; CAI; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase.
Sequence
MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 50mM NaCl, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week.
For long term storage, aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
29.2 kDa (260aa), confirmed by MALDI-TOF.
3D Structure
ModBase 3D Structure for P00918
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Domain
1-260aa
Biological Activity
Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.
Tag
Non-tagged

Product Categories/Family for CA2 active protein

Applications Tested/Suitable for CA2 active protein
SDS-PAGE, Enzyme Activity

SDS-Page of CA2 active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
CA2 Active Protein – Cat# MBS203326

Product References and Citations for CA2 active protein

Hu PY., et al. (1992). Hum Mutat. 1 (4):288-92 ; Venta PJ., et al. (1991). Am J Hum Genet. 49 (5):1082-90;

Other Notes

Small volumes of CA2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA2 purchase

MBS203326 is an active protein targeting CA2. Also known as Carbonic anhydrase 2, 1-260aa, Human, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carbonic anhydrase 2 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
CA2
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
UniProt Gene Name
CA2
UniProt Synonym Gene Names
CAC; CA-II
NCBI Protein Information
carbonic anhydrase 2

NCBI Summary for CA2

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
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UniProt Comments for CA2

Essential for bone resorption and osteoclast differentiation (). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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